Periosteum as a source of mesenchymal stem cells: the effects of TGF-β3 on chondrogenesis
| Autor: | Ibsen Bellini Coimbra, Angélica Rossi Sartori, Marcio Amaral Camargo Pedro, Cristiane Sampaio de Mara, Adriana S. S. Duarte, André L. L Andrade |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Adult
Cellular differentiation Blotting Western Type II collagen Gene Expression Biology Flow cytometry Transforming Growth Factor beta3 Western blot Periosteum medicine Humans High-density Culture Collagen Type II Cells Cultured Analysis of Variance medicine.diagnostic_test Monolayer Culture Reverse Transcriptase Polymerase Chain Reaction Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells General Medicine Middle Aged Chondrogenesis Cell biology Basic Research medicine.anatomical_structure Immunology Stem cell |
| Zdroj: | Clinics, Volume: 66, Issue: 3, Pages: 487-492, Published: 2011 Clinics |
| Popis: | INTRODUCTION: Numerous experimental efforts have been undertaken to induce the healing of lesions within articular cartilage by re-establishing competent repair tissue. Adult mesenchymal stem cells have attracted attention as a source of cells for cartilage tissue engineering. The purpose of this study was to investigate chondrogenesis employing periosteal mesenchymal cells. METHODS: Periosteum was harvested from patients who underwent orthopedic surgeries. Mesenchymal stem cells were characterized through flow cytometry using specific antibodies. The stem cells were divided into four groups. Two groups were stimulated with transforming growth factor β3 (TGF-β3), of which one group was cultivated in a monolayer culture and the other was cultured in a micromass culture. The remaining two groups were cultivated in monolayer or micromass cultures in the absence of TGF-β3. Cell differentiation was verified through quantitative reverse transcription-polymerase chain reaction (RT-PCR) and using western blot analysis. RESULT: In the groups cultured without TGF-β3, only the cells maintained in the micromass culture expressed type II collagen. Both the monolayer and the micromass groups that were stimulated with TGF-β3 expressed type II collagen, which was observed in both quantitative RT-PCR and western blot analysis. The expression of type II collagen was significantly greater in the micromass system than in the monolayer system. CONCLUSION: The results of this study demonstrate that the interactions between the cells in the micromass culture system can regulate the proliferation and differentiation of periosteal mesenchymal cells during chondrogenesis and that this effect is enhanced by TGF-β3. |
| Databáze: | OpenAIRE |
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