RNAi-Mediated Functional Analysis of Bursicon Genes Related to Adult Cuticle Formation and Tanning in the Honeybee, Apis mellifera
| Autor: | Claudineia Pereira Costa, Juliana Martins, Tiago Falcon, Márcia Maria Gentile Bitondi, Moysés Elias-Neto, Rodrigo Pires Dallacqua |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Life Cycles Invertebrate Hormones Physiology lcsh:Medicine Gene Expression Molting Receptors G-Protein-Coupled chemistry.chemical_compound 0302 clinical medicine Cyclic AMP Medicine and Health Sciences Wings Animal lcsh:Science Bursicon Gene knockdown Multidisciplinary integumentary system Drosophila Melanogaster Metamorphosis Biological Integumentary system Gene Expression Regulation Developmental Phylogenetic Analysis Animal Models HORMÔNIOS Bees Cell biology Insects Ecdysis Gene Knockdown Techniques RNA Interference Drosophila Invertebrate hormone Drosophila melanogaster Anatomy Integumentary System Honey Bees Research Article animal structures Arthropoda Cuticle Biology Research and Analysis Methods 03 medical and health sciences Model Organisms Botany Genetics Animals Molecular Biology Techniques Molecular Biology Ecdysteroid Molecular Biology Assays and Analysis Techniques lcsh:R fungi Organisms Ecdysteroids Biology and Life Sciences Pupae biology.organism_classification Invertebrates Hymenoptera 030104 developmental biology chemistry lcsh:Q Physiological Processes 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | PLoS ONE Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP PLoS ONE, Vol 11, Iss 12, p e0167421 (2016) |
| ISSN: | 1932-6203 |
| Popis: | Bursicon is a heterodimeric neurohormone that acts through a G protein-coupled receptor named rickets (rk), thus inducing an increase in cAMP and the activation of tyrosine hydroxylase, the rate-limiting enzyme in the cuticular tanning pathway. In insects, the role of bursicon in the post-ecdysial tanning of the adult cuticle and wing expansion is well characterized. Here we investigated the roles of the genes encoding the bursicon subunits during the adult cuticle development in the honeybee, Apis mellifera. RNAi-mediated knockdown of AmBurs α and AmBurs β bursicon genes prevented the complete formation and tanning (melanization/sclerotization) of the adult cuticle. A thinner, much less tanned cuticle was produced, and ecdysis toward adult stage was impaired. Consistent with these results, the knockdown of bursicon transcripts also interfered in the expression of genes encoding its receptor, AmRk, structural cuticular proteins, and enzymes in the melanization/sclerotization pathway, thus evidencing roles for bursicon in adult cuticle formation and tanning. Moreover, the expression of AmBurs α, AmBurs β and AmRk is contingent on the declining ecdysteroid titer that triggers the onset of adult cuticle synthesis and deposition. The search for transcripts of AmBurs α, AmBurs β and candidate targets in RNA-seq libraries prepared with brains and integuments strengthened our data on transcript quantification through RT-qPCR. Together, our results support our premise that bursicon has roles in adult cuticle formation and tanning, and are in agreement with other recent studies pointing for roles during the pharate-adult stage, in addition to the classical post-ecdysial ones. |
| Databáze: | OpenAIRE |
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