Isolation and characterisation of sex-specific transcripts from Oesophagostomum dentatum by RNA arbitrarily-primed PCR
| Autor: | Peter Nansen, Robin B. Gasser, C. M. Christensen, Susan E Newton, Peter R. Boag, Niels-Peter Hansen |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Male
Transcription Genetic Swine Sequence analysis Molecular Sequence Data Polymerase Chain Reaction law.invention law Gene expression Animals RNA Messenger Northern blot Molecular Biology Strongylida Caenorhabditis elegans Polymerase chain reaction Expressed Sequence Tags Genetics Sex Characteristics Expressed sequence tag biology Gene Expression Profiling RNA Sequence Analysis DNA Blotting Northern biology.organism_classification Molecular biology Oesophagostomum Female Parasitology RNA Helminth Oesophagostomiasis |
| Zdroj: | Monash University |
| ISSN: | 0166-6851 |
| DOI: | 10.1016/s0166-6851(00)00217-6 |
| Popis: | In light of the lack of molecular data on the sexual differentiation, maturation and interaction of parasitic nematodes of livestock, the present study investigated sex-specific gene expression in the nodule worm, Oesophagostomum dentatum (Strongylida). Using the technique of RNA arbitrarily-primed polymerase chain reaction (RAP-PCR), 31 expressed sequence tags (ESTs) differentially-displayed between the sexes were cloned. Northern blot analysis proved ten ESTs to be expressed exclusively in males (adults and fourth-stage larvae), while two were expressed solely in female stages. None of the ESTs were expressed in infective third-stage larvae. Sequence analysis and subsequent database searches revealed two male-specific ESTs to have significant similarity to Caenorhabditis elegans (predicted) proteins, a protein containing an EGF-like cysteine motif and a serine/threonine phosphatase. Another two male-specific ESTs had similarity to non-nematode sequences. The two female-specific ESTs had similarity to vitellogenin-5 and endonuclease III (predicted) from C. elegans. The remaining ESTs had no similarity to any nucleic acid or protein sequences contained in the databases. The isolation and characterisation of sex-specific ESTs from O. dentatum provides a unique opportunity for studying the reproductive biology of parasitic nematodes at the molecular level, with a view toward novel approaches for parasite control. |
| Databáze: | OpenAIRE |
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