Increased sensitivity of fat cell adenylate cyclase to stimulatory agonists during fasting is not related to impaired inhibitory coupling system
| Autor: | Brigitte Agli, Yves Giudicelli, Danièle Lacasa |
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| Rok vydání: | 1986 |
| Předmět: |
Male
medicine.medical_specialty Time Factors GTP' Biophysics Adenylate kinase Adipose tissue Biology Inhibitory postsynaptic potential Pertussis toxin Niacin Biochemistry Cyclase Hormonal sensitivity GTP-Binding Proteins Structural Biology Internal medicine Cyclic AMP Genetics medicine Adenylate cyclase Animals Virulence Factors Bordetella Receptor Molecular Biology Colforsin Rats Inbred Strains Fasting Cell Biology Ni protein (Rat adipocyte) Rats Nicotinic acid Nicotinic agonist Endocrinology Adipose Tissue Pertussis Toxin Starvation Phenylisopropyladenosine Adenylate Cyclase Toxin Guanosine Triphosphate Adenylyl Cyclases |
| Zdroj: | FEBS Letters. 202:260-266 |
| ISSN: | 0014-5793 |
| Popis: | In rat adipocytes, inhibition of the forskolin-stimulated cyclic AMP response by nicotinic acid and N6-phenylisopropyladenosine was unaltered by a 72 h fasting. Under assay conditions favouring inhibition, basal and forskolin-stimulated adenylate cyclase responses to inhibition by GTP and nicotinic acid were also unimpaired by fasting. Under the same conditions, however, low GTP concentrations elicited a clear activatory effect in membranes from fasted but not from fed rats. Fasting failed to alter the incorporation of [32P]ADP ribose into the αi-subunit of Ni and the attenuation of nicotinic acid inhibitory action that are both induced by pertussis toxin. These results, demonstrating unimpaired inhibitory control of adenylate cyclase in starved rat adipocytes, suggest that the permissive effect of fasting on the action of stimulatory receptor agonists in fat cells reflects a specific increase in the activity of the adenylate cyclase stimulatory coupling system. |
| Databáze: | OpenAIRE |
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