Development and Molecular Characterization of Novel Polymorphic Genomic DNA SSR Markers in Lentinula edodes
| Autor: | Rhim Ryoo, Hwa-Yong Lee, Han-Gyu Ko, Donghwan Shim, Jong-Wook Chung, Kang-Hyeon Ka, Myungkil Kim, Chang-Duck Koo, Hojin Ryu, Suyun Moon |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Genetics Genetic diversity Dendrogram UPGMA food and beverages genetic diversity Biology gDNA-SSR biology.organism_classification Microbiology Genome Loss of heterozygosity 03 medical and health sciences genomic DNA Research Note 030104 developmental biology Infectious Diseases Lentinula Lentinula edodes Allele frequency |
| Zdroj: | Mycobiology |
| ISSN: | 2092-9323 1229-8093 |
| Popis: | Sixteen genomic DNA simple sequence repeat (SSR) markers of Lentinula edodes were developed from 205 SSR motifs present in 46.1-Mb long L. edodes genome sequences. The number of alleles ranged from 3-14 and the major allele frequency was distributed from 0.17-0.96. The values of observed and expected heterozygosity ranged from 0.00-0.76 and 0.07-0.90, respectively. The polymorphic information content value ranged from 0.07-0.89. A dendrogram, based on 16 SSR markers clustered by the paired hierarchical clustering' method, showed that 33 shiitake cultivars could be divided into three major groups and successfully identified. These SSR markers will contribute to the efficient breeding of this species by providing diversity in shiitake varieties. Furthermore, the genomic information covered by the markers can provide a valuable resource for genetic linkage map construction, molecular mapping, and marker-assisted selection in the shiitake mushroom. |
| Databáze: | OpenAIRE |
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