Sensitive method for endotoxin determination in nanomedicinal product samples
| Autor: | Paul Schwillens, Eric R. Gremmer, Louis van Bloois, Wim H. de Jong, Geertsma Re, Peter H. J. Keizers, Christina Giannakou, Kukka Aimonen, Rob J. Vandebriel, Julia Catalán, Margriet V. D. Z. Park |
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| Přispěvatelé: | RS: GROW - R1 - Prevention, Promovendi ODB, Toxicogenomics |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Lipopolysaccharides
Dendrimers DOXORUBICIN BACTERIAL LIPOPOLYSACCHARIDES Biomedical Engineering interference GAS-CHROMATOGRAPHY Medicine (miscellaneous) Bioengineering 02 engineering and technology Development Mass spectrometry Ferric Compounds fatty acids TOXICITY 03 medical and health sciences DELIVERY Tandem Mass Spectrometry SYSTEMS LAL assay NANOPARTICLES General Materials Science Particle Size Chromatography High Pressure Liquid 030304 developmental biology Titanium NANOMATERIALS 0303 health sciences Chromatography Chemistry lipopolysaccharide Membrane Proteins Cerium MASS-SPECTROMETRY 021001 nanoscience & nanotechnology nanomedicine Nanostructures CONTAMINATION Limulus amebocyte lysate Liposomes Biological Assay Ultra high performance 0210 nano-technology |
| Zdroj: | Nanomedicine, 14(10), 1231-1246. Future Medicine Ltd. |
| ISSN: | 1743-5889 |
| DOI: | 10.2217/nnm-2018-0339 |
| Popis: | Aim: Nanomaterials and nanomedicinal products tend to interfere with various commonly used assays, including regulatory required endotoxin detection methods for medicines. We developed a method to quantify endotoxin levels that is compatible with nanomaterials and nanomedicinal products. Materials & methods: The method is based on measuring endotoxin indirectly via 3-hydroxylated fatty acids of lipid-A, using Ultra High Performance Liquid Chromatography coupled with mass spectrometry. The outcome was related to results of the commonly used Limulus Amebocyte Lysate method. Results: The ultra high performance liquid chromatography coupled with mass spectrometry method has clear advantages compared with other endotoxin determination assays; particularly the absence of nanospecific interference. Conclusion: The method is sensitive, straightforward and accurate in determining and quantifying endotoxin in nanomedicinal product samples. |
| Databáze: | OpenAIRE |
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