Enterobacterial tumor colonization in mice depends on bacterial metabolism and macrophages but is independent of chemotaxis and motility
| Autor: | Tobias A. Oelschlaeger, Aladar A. Szalay, Achim Tresch, Carolin Seubert, Jochen Stritzker, Nico van Rooijen, Andreas Götz, Ivaylo Gentschev, Philip J. Hill, Stephanie Weibel |
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| Přispěvatelé: | Molecular cell biology and Immunology, CCA - Immuno-pathogenesis |
| Rok vydání: | 2010 |
| Předmět: |
Salmonella typhimurium
Microbiology (medical) Motility medicine.disease_cause Microbiology Amino Acids Aromatic Mice Neoplasms Escherichia coli medicine Animals Macrophage Mice Inbred BALB C Tumor microenvironment biology Chemotaxis Macrophages General Medicine Mononuclear phagocyte system biology.organism_classification Bacterial Load Biosynthetic Pathways Infectious Diseases Salmonella enterica Mutation Female Locomotion Bacteria |
| Zdroj: | Stritzker, J, Weibel, S, Seubert, C, Gotz, A, Tresch, A, van Rooijen, N, Oelschlaeger, T A, Hill, P J, Gentschev, I & Szalay, A A 2010, ' Enterobacterial tumor colonization in mice depends on bacterial metabolism and macrophages but is independent of chemotaxis and motility ', International Journal of Medical Microbiology, vol. 300, no. 7, pp. 449-456 . https://doi.org/10.1016/j.ijmm.2010.02.004 International Journal of Medical Microbiology, 300(7), 449-456. Urban und Fischer Verlag GmbH und Co. KG |
| ISSN: | 1438-4221 |
| DOI: | 10.1016/j.ijmm.2010.02.004 |
| Popis: | Despite promising results and increasing attention in bacterial cancer therapy, surprisingly little is known about initial tumor colonization and the interaction between bacteria and surrounding tumor tissue. Here, we analyzed the role of chemotaxis, motility, and metabolism both in Escherichia coli and Salmonella enterica serovar Typhimurium strains upon intravenous injection into tumor-bearing mice. In contrast to previous models, we found that chemotaxis and motility do not play a significant role in tumor colonization and bacterial distribution within the tumor. Rather, the whole colonization and intratumoral migration process seems to be a passive mechanism that is influenced by the reticuloendothelial system of the host, by the tumor microenvironment and by the bacterial metabolism. These conclusions were supported by experimental data demonstrating that disruption of the basic branch of the aromatic amino acid biosynthetic pathway and depletion of macrophages, in contrast to flagellar mutations, led to significant changes in bacterial accumulation in tumors of live mice. |
| Databáze: | OpenAIRE |
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