Monitoring Physiological Changes in Haloarchaeal Cell during Virus Release
| Autor: | Dennis H. Bamford, Hanna M. Oksanen, Rimantas Daugelavičius, Julija Svirskaitė |
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| Přispěvatelé: | Institute of Biotechnology, Molecular Principles of Viruses, Biosciences, Structure of the Viral Universe |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Archaeal Viruses HALOARCULA-HISPANICA viruses Cell lcsh:QR1-502 LIPOPHILIC ANIONS Cell Count lcsh:Microbiology spindle-shaped virus His1 pleomorphic virus His2 Adenosine Triphosphate HYPERSALINE ENVIRONMENTS 1183 Plant biology microbiology virology Virus Release CAPSID PROTEINS Viral Load Halophile 3. Good health HALOPHILIC ARCHAEA Sphaerolipoviridae TURRETED ICOSAHEDRAL VIRUS Infectious Diseases medicine.anatomical_structure Spectrophotometry STRANDED-DNA-BACTERIOPHAGE Haloarcula hispanica icosahedral membrane-containing virus SH1 icosahedral tailed virus HHTV-1 potentiometry virus life cycle Pleolipoviridae Boron Compounds Cell Survival Biology Virus Article Microbiology 03 medical and health sciences Oxygen Consumption Viral life cycle Virology medicine Viable cell HOST INTERACTIONS Haloarcula ARCHAEAL VIRUS 030104 developmental biology PHAGE-PHI-H |
| Zdroj: | Viruses; Volume 8; Issue 3; Pages: 59 Viruses Viruses, Vol 8, Iss 3, p 59 (2016) |
| ISSN: | 1999-4915 |
| DOI: | 10.3390/v8030059 |
| Popis: | The slow rate of adsorption and non-synchronous release of some archaeal viruses have hindered more thorough analyses of the mechanisms of archaeal virus release. To address this deficit, we utilized four viruses that infect Haloarcula hispanica that represent the four virion morphotypes currently known for halophilic euryarchaeal viruses: (1) icosahedral internal membrane-containing SH1; (2) icosahedral tailed HHTV-1; (3) spindle-shaped His1; and (4) pleomorphic His2. To discern the events occurring as the progeny viruses exit, we monitored culture turbidity, as well as viable cell and progeny virus counts of infected and uninfected cultures. In addition to these traditional metrics, we measured three parameters associated with membrane integrity: the binding of the lipophilic anion phenyldicarbaundecaborane, oxygen consumption, and both intra- and extra-cellular ATP levels. |
| Databáze: | OpenAIRE |
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