A comparative investigation on the in vitro anti-inflammatory, antioxidant and antimicrobial potentials of subextracts from the aerial parts of Daphne oleoides Schreb. subsp. oleoides
| Autor: | İrem Atay Balkan, Galip Akaydin, Hacer Tuğba Doğan, Erdem Yesilada, Inci Deniz, Turgut Taşkın |
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| Přispěvatelé: | Balkan, İ.A., Taşkın, T., Doğan, H.T., Deniz, İ., Akaydın, G., Yeşilada, Erdem, Yeditepe Üniversitesi |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Daphne Oleoides Subsp Oleoides Antioxidant DPPH medicine.medical_treatment Flavonoid Ethyl acetate 01 natural sciences Anti-Inflammatory 03 medical and health sciences chemistry.chemical_compound medicine Organic chemistry Fatty acids chemistry.chemical_classification ABTS Traditional medicine biology Fatty Acids Fatty acid biology.organism_classification Daphne oleoides 0104 chemical sciences 010404 medicinal & biomolecular chemistry 030104 developmental biology chemistry Daphne oleoides subsp. oleoides Thymelaeaceae Antimicrobial Anti-inflammatory Phenolics Agronomy and Crop Science |
| Zdroj: | Industrial Crops and Products. 95:695-703 |
| ISSN: | 0926-6690 |
| DOI: | 10.1016/j.indcrop.2016.11.038 |
| Popis: | WOS: 000390621600079 Daphne oleoides Schreb. subsp. oleoides is an evergreen shrub which is known for its folkloric usage against various ailments such as arthritic and infectious diseases. The aim of the present study was to comparatively investigate the in vitro anti-inflammatory (nitric oxide inhibitory effects), antioxidant (DPPH center dot,ABTS(center dot+) and FRAP tests) and antimicrobial activities of the extract/subextracts from the aerial parts of D. oleoides Schreb. subsp. oleoides (Thymelaeaceae). The powdered aerial parts were first extracted with methanol and then partitioned into five subextracts (n-hexane, dichloromethane (CH2Cl2), ethyl acetate (EtOAC), n-butanol and remaining water) by succesive solvent extractions. The chemical compositions of the extracts were compared with regard to their total phenolic, total flavonoid contents and fatty acid compositions. The CH2Cl2 and n-hexane extracts exerted the highest inhibitory effect on NO production (61.67 +/- 13.97% inhibition at 50 mu g/ml and 67.91 +/- 11.17% inhibition at 100 mu g/ml, respectively) of LPS-induced Raw 264.7 cells. The EtOAC subextract showed the highest antioxidant effect evaluated by DPPH center dot (IC50: 0.35 +/- 0.12 mg/ml) and FRAP (4.54 +/- 10.01 mM FeSO4/mg extract) tests. The CH2Cl2 subextract was shown to contain the highest levels of flavonoids (0.837 +/- 10.035 mg QUE/mg extract) and was also found to be rich in phenolics (1.063 +/- 10.011 mg GAE/mg extract). Fatty acid analysis of the n-hexane extract revealed hexadecahoic acid and 9,15-octodecadienoic acids to be the major components. The n-hexane and CH2Cl2 subextracts exerted the'highest antimicrobial potential. |
| Databáze: | OpenAIRE |
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