Diversity of Culturable Bacteria Isolated From the Feces of Wild Anopheles darlingi (Diptera: Culicidae) Mosquitoes From the Brazilian Amazon
| Autor: | Najla Benevides Matos, Alexandre Almeida e Silva, Gabriel Eduardo Melim Ferreira, Andrelisse Arruda, Antonio dos Santos Júnior, Rodrigo G. Stábeli, Luiz S. Ozaki, Tatiane S Carvalho |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Mosquito Control
Serratia Zoology Paratransgenesis Mosquito Vectors Serratia liquefaciens Feces 03 medical and health sciences chemistry.chemical_compound RNA Ribosomal 16S Anopheles parasitic diseases Animals Phylogeny 030304 developmental biology 0303 health sciences Bacteria General Veterinary biology 030306 microbiology Microbiota 16S ribosomal RNA biology.organism_classification Malaria Infectious Diseases Biological Control Agents chemistry Genes Bacterial Insect Science Parasitology Metagenomics Brazil Nutrient agar |
| DOI: | 10.5281/zenodo.6363602 |
| Popis: | Microorganisms living in the midgut of Anopheles mosquitoes have been studied to fight vector-borne diseases, such as malaria. Studies on the microbiota of the Neotropical Anopheles darlingi, the most important Brazilian vector for malaria, have been reported for the same purpose. Our aims were to isolate and identify culturable bacteria from An. darlingi mosquito guts through their feces and to estimate the species richness and the frequency distribution of the sampled bacteria. Sixty wild females of An. darlingi mosquitoes were captured at two rural locations, near Porto Velho, Rondônia, Brazil. Bacteria were isolated from mosquito feces, which were collected using cages which permit the collection of feces on LB nutrient agar plates. Sixty bacterial colonies were isolated and stored in glycerol at −80°C. Bacteria were identified by sequencing their 16S rRNA gene obtained using PCR and Sanger sequencing. To aid in species identification, MALDI-TOF, VITEK2, and BBL Crystal were used as complementary protocols. The sequences obtained from the 60 bacterial isolates were compared to sequences deposited in GenBank (NCBI) using BLAST. Homology greater than 97% between the query and the subject was used as the criteria for assigning the identity of each isolate. Fourteen species from eight different genera were identified among the 60 isolates. The most frequent species were Serratia liquefaciens (20%) and Serratia marcescens (15%). Due to their established apathogenicity and according to previous studies, we suggest Serratia and Pantoea species as suitable for paratransgenesis development to fight malaria in Brazilian Amazon. This is a pre-copyedited, author-produced PDF of an article accepted for publication in Journal of Medical Entomology following peer review. The version of record [Diversity of Culturable Bacteria Isolated From the Feces of Wild Anopheles darlingi (Diptera: Culicidae) Mosquitoes From the Brazilian Amazon. Journal of Medical Entomology 58, 4 p1900-1907 (2021)] is available online at: https://doi.org/10.1093/jme/tjab028. Deposited by shareyourpaper.org and openaccessbutton.org. We've taken reasonable steps to ensure this content doesn't violate copyright. However, if you think it does you can request a takedown by emailing help@openaccessbutton.org. |
| Databáze: | OpenAIRE |
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