Regulation of taurine in OTA-induced apoptosis and autophagy
| Autor: | Dandan Liu, Cuiling Pan, Xingxiang Chen, Kehe Huang, Xinru Mao, Fang Gan, Kai Liu, Guannan Le, Hu Li |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0303 health sciences Taurine Chemistry Cell Survival 010604 marine biology & hydrobiology 030302 biochemistry & molecular biology Autophagy Cellular homeostasis Apoptosis Pharmacology Toxicology 01 natural sciences Ochratoxins Nephrotoxicity 03 medical and health sciences chemistry.chemical_compound Annexin Toxicity Animals Homeostasis |
| Zdroj: | Toxicon : official journal of the International Society on Toxinology. 181 |
| ISSN: | 1879-3150 |
| Popis: | Ochratoxin A (OTA), one of the most deleterious mycotoxins, could cause a variety of toxicological effects especially nephrotoxicity in animals and humans. Taurine, a wide-distributed cytoprotective amino acid, plays an important role as a basic factor for maintaining cellular integrity homeostasis. However, the potential effect of taurine in OTA-induced nephrotoxicity remains unknown. In the present study, we demonstrated that OTA treatment at 4.0–8.0 μM increased apoptosis in PK-15 cells as shown by increased the ratio of apoptosis and protein expression of Bax and cleaved-caspase-3, decreased protein expression of Bcl-2. Meantime, OTA treatment triggered autophagy, as indicated by markedly increased the protein expression of LC3-II and fluorescence intensity of GFP-LC3 dots. Taurine supplementation decreased OTA-induced cytotoxicity and attenuated apoptosis as shown by the decreased Annexin V/PI staining and the decreased expression of apoptosis-related proteins including Bax and caspase-3. Meanwhile, taurine attenuated OTA-induced autophagy by decreased the protein expression of LC3-II and fluorescence intensity of GFP-LC3 dots to maintain cellular homeostasis. In conclusion, taurine treatment could alleviate OTA-induced apoptosis and inhibit the triggered autophagy in PK-15 cells. Our study provides supportive data for the potential roles of taurine in reducing OTA-induced renal toxicity. |
| Databáze: | OpenAIRE |
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