Molecular genotyping of Indian blood group system antigens in Indian blood donors
| Autor: | Harita Gogri, Pranali Pitale, Manisha Madkaikar, Swati Kulkarni |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Antiserum
Hemagglutination Genotype Blood Donors Hematology 030204 cardiovascular system & hematology Biology Virology 03 medical and health sciences 0302 clinical medicine Antigen Asian People biology.protein Blood Group Antigens Humans Allele Antibody Indian blood group system Genotyping Gene 030215 immunology |
| Zdroj: | Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis. 57(3) |
| ISSN: | 1473-0502 |
| Popis: | Background Haemagglutination has been the gold standard for defining the blood group status. However, these tests depend upon the availability of specific and reliable antisera. Potent antisera for extended phenotyping are very costly, weakly reacting or available in limited stocks and unavailable for some blood group systems like Indian, Dombrock, Coltan, Diego etc. The Indian blood group system consists of two antithetical antigens, Ina and Inb. The Ina /Inb polymorphism arises from 252C > G missense mutation in the CD44 gene. This knowledge has allowed the development of molecular methods for genotyping IN alleles. Material and methods Blood samples were collected from 715 blood donors from Mumbai. DNA was extracted using phenol-chloroform method and genotyping for Indian (Ina/IN*01, Inb /IN*02) blood group alleles was done by Sequence Specific PCR. Results Seventeen donors among 715 were heterozygous for Ina antigen i.e. In (a+b+). The Ina antigen positivity was confirmed serologically, using anti-Ina prepared in-house and the genotype-phenotype results were concordant. The frequency of Ina (2.37%) was higher than Caucasians and comparable to those reported among Indians of Bombay. Conclusion This is the first study reporting molecular screening of Indian blood group antigens in Indian population. The frequency of Ina and Inb antigens was found to be 2.37% and 100% respectively. Red cells of Ina positive donors can be used as in-house reagent red cells for screening and identification of corresponding antibodies. Thus, DNA based methods will help in large scale screening of donors to identify rare blood groups, when commercial antisera are unavailable. |
| Databáze: | OpenAIRE |
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