Enhanced activity of vancomycin by encapsulation in hybrid magnetic nanoparticles conjugated to a cell-penetrating peptide
| Autor: | Zoleikha Hajizadeh, Mohammad Reza Ahghari, Michael R. Hamblin, Ye Tian, Ali Maleki, Reza Taheri-Ledari, Wenjie Zhang, Ehsan Zolfaghari |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Staphylococcus aureus
Carrier system Drug Compounding Peptide Cell-Penetrating Peptides Microbial Sensitivity Tests 02 engineering and technology Conjugated system 010402 general chemistry medicine.disease_cause 01 natural sciences Polyvinyl alcohol law.invention chemistry.chemical_compound Vancomycin Confocal microscopy law Escherichia coli medicine General Materials Science Magnetite Nanoparticles chemistry.chemical_classification Drug Carriers Chemistry technology industry and agriculture 021001 nanoscience & nanotechnology Combinatorial chemistry Anti-Bacterial Agents 0104 chemical sciences Cell-penetrating peptide Magnetic nanoparticles 0210 nano-technology |
| Zdroj: | Nanoscale. 12:3855-3870 |
| ISSN: | 2040-3372 2040-3364 |
| DOI: | 10.1039/c9nr09687f |
| Popis: | We describe a novel antibiotic delivery system based on magnetic nanoparticles (NPs) conjugated to a cell-penetrating peptide (CPP). Silica-coated iron oxide NPs were produced via a co-deposition method, and coated by a polyvinyl alcohol (PVA) polymeric network via physicochemical binding. Vancomycin (VAN) was then entrapped into this PVA network. A hexapeptide sequence Gly-Ala-Phe-Pro-His-Arg, was synthesized in the solid phase and then conjugated onto the surface of the magnetic NPs. The drug ratio incorporation into the carrier system and drug release were monitored through precise analysis. Confocal microscopy showed that the NPs could be internalized into Staphylococcus aureus and Escherichia coli bacterial cells. The antimicrobial effects of VAN were significantly enhanced by this system with a low dosage of VAN. Advantages include rapid targeted-drug delivery process, drug dose reduction, and equal effects on both Gram-positive and Gram-negative bacteria. |
| Databáze: | OpenAIRE |
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