Molecular and biological characteristics of echovirus 22, a representative of a new picornavirus group
| Autor: | Farideh Ghazi, Mahboob Khan, Glyn Stanway, Timo Hyypiä, Nisse Kalkkinen, M. Smyth, M. Roivainen, O. Meurman |
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| Rok vydání: | 1994 |
| Předmět: |
Echovirus
Picornavirus Sequence analysis viruses Molecular Sequence Data Immunology Picornaviridae medicine.disease_cause Microbiology Genome Virus Capsid Virology medicine Animals Humans Amino Acid Sequence Peptide sequence Phylogeny Genetics chemistry.chemical_classification Sequence Homology Amino Acid biology virus diseases biochemical phenomena metabolism and nutrition biology.organism_classification Peptide Fragments Enterovirus B Human Amino acid chemistry Insect Science Research Article |
| Zdroj: | Journal of Virology. 68:8232-8238 |
| ISSN: | 1098-5514 0022-538X |
| Popis: | Recent sequence analysis revealed that the human pathogen echovirus 22 (EV22) is genetically distant from all the other picornaviruses studied to date (T. Hyypiä, C. Horsnell, M. Maaronen, M. Khan, N. Kalkkinen, P. Auvinen, L. Kinnunen, and G. Stanway, Proc. Natl. Acad. Sci. USA 89:8847-8851, 1992). We have further characterized the biological properties of the virus and show here that the virion has properties similar to those of other picornaviruses. However, the protein composition is unique, in that most copies of one of the three major capsid proteins, VP0, do not undergo the further processing to VP2 and VP4 observed during the maturation of the virus in previously studied picornaviruses. Alignment of the capsid protein sequences with those of other picornaviruses revealed, furthermore, that the VP3 polypeptide contains an apparent insertion of approximately 25 amino acids at its amino terminus. An arginine-glycine-aspartic acid (RGD) motif is found in VP1, and by using synthetic peptides, it was shown that this sequence plays a role in cell surface receptor recognition. Finally, EV23 was shown to share remarkable identity with EV22 in certain parts of the genome and also belongs to this previously unrecognized picornavirus group. |
| Databáze: | OpenAIRE |
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