Caspase-dependent cleavage regulates protein levels of Epstein-Barr virus-derived latent membrane protein 1
| Autor: | Tadashi Matsuda, Osamu Ikeda, Yuichi Kitai, Kenji Oritani, Eri Kawanishi, Ryuta Muromoto, Teruhito Yasui, Koki Hirashima, Yosuke Hatano, Shigeyuki Kon, Sumihito Togi |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
STAT3 Transcription Factor
0301 basic medicine Herpesvirus 4 Human Genes Viral caspase Biophysics Gene Expression Caspase 3 medicine.disease_cause Biochemistry Virus Viral Matrix Proteins 03 medical and health sciences Enzyme activator 0302 clinical medicine EBV Structural Biology Gene expression otorhinolaryngologic diseases Genetics medicine Humans RNA Small Interfering Molecular Biology LMP1 Caspase degradation Viral matrix protein biology NF-kappa B NF-kappa B activation Cell Biology NFKB1 Caspase Inhibitors Epstein–Barr virus Recombinant Proteins Protein Structure Tertiary Cell biology Enzyme Activation stomatognathic diseases 030104 developmental biology 030220 oncology & carcinogenesis Host-Pathogen Interactions Proteolysis biology.protein Oligopeptides HeLa Cells |
| Zdroj: | FEBS Letters. 590:808-818 |
| ISSN: | 0014-5793 |
| DOI: | 10.1002/1873-3468.12119 |
| Popis: | Epstein-Barr virus (EBV)-encoded latent membrane protein-1 (LMP1) plays pathogenic roles in EBV-related diseases. Thus, host cells employ several mechanisms to regulate LMP1 functions, and we previously reported possible regulation by signal transducing adaptor protein-2 as well as BS69. Here, we found that caspase-3 mainly degraded LMP1 proteins in HeLa cells, leading to decreased NF-kappa B and STAT3 activation. Caspase-3 cleaved the consensus DNTD sequences in the CTAR3 region of LMP1. Of importance, LMP1 expression strongly enhanced caspase-3 activity. Taken together, the reduction of LMP1 protein levels by caspases is likely to be a newly identified host defense against EBV infection. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text