Capillary gas chromatographic—electron-capture assay for the aldose reductase inhibitor imirestat in lens and plasma
| Autor: | Bette A. McCue, R. K. Brazzell, Young Han Park, J.J. Boltralik |
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| Rok vydání: | 1991 |
| Předmět: |
Detection limit
Fluorenes Aldose reductase Chromatography Gas Chromatography Chemistry Administration Topical Hydantoins Extraction (chemistry) Lens (geology) General Chemistry Aldose reductase inhibitor Plasma Electron capture detector chemistry.chemical_compound Aldehyde Reductase Lens Crystalline medicine Animals Humans Rabbits Gas chromatography Derivatization medicine.drug |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 565:255-264 |
| ISSN: | 0378-4347 |
| DOI: | 10.1016/0378-4347(91)80388-s |
| Popis: | A sensitive and selective gas chromatographic-electron-capture assay was developed for the determination of the aldose reductase inhibitor imirestat in lens and plasma. The method involves solid-phase extraction of drug and internal standard from the plasma specimen or lens sample homogenate using "Baker"-10 SPE extraction columns followed by derivatization with pentafluorobenzyl bromide and further purification. Derivatives of drug and internal standard were separated on a fused-silica capillary column and analyzed using a 63Ni electron-capture detector. The limit of detection was 2.5 ng per lens or ml of plasma. The method was used to evaluate the pharmacokinetics of imirestat in human subjects and to quantitate imirestat in animal lens tissue following topical ocular administration. |
| Databáze: | OpenAIRE |
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