Expression of neural cell adhesion molecule L1 in human lung cancer cell lines
| Autor: | Tohru Masuda, Hiromi Wada, Hiroshi Masutani, Keizo Furuke, Masahiro Katayama, Kazumasa Takeda, Kazuhiro Ishii, Akihiko Iwamatsu |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Lung Neoplasms
L1 Physiology medicine.drug_class Cell Adhesion Molecules Neuronal Biology Monoclonal antibody Chromatography Affinity Flow cytometry Mice Neuroblastoma Antigen medicine Tumor Cells Cultured Animals Humans Molecular Biology Mice Inbred BALB C Hybridomas Membrane Glycoproteins medicine.diagnostic_test Antibodies Monoclonal Cell Biology General Medicine Molecular biology Precipitin Tests Cell biology Cell culture Fluorescent Antibody Technique Direct biology.protein Electrophoresis Polyacrylamide Gel Antibody Clone (B-cell biology) Multiple Myeloma Leukocyte L1 Antigen Complex Sequence Alignment Sequence Analysis Immunostaining Spleen |
| Zdroj: | Cell structure and function. 22(5) |
| ISSN: | 0386-7196 |
| Popis: | To investigate neuron-specific antigens, hybridoma cells were produced between mouse spleen cells immunized with human neuroblastoma cells (IMR-32) and mouse myeloma cells. 247 hybridoma clones were harvested and one of them was further cultured for recloning. Eventually, one hybridoma clone was obtained and its antibody was designated N-A8. The characteristics of this antibody were determined by immunostaining and flow cytometry. First, the antibody recognized the surface antigens of IMR-32 cells. Second, unexpectedly, N-A8 was reactive not only with human neuroblastoma cell lines but also with human lung cancer cell lines. As analyzed by immunoprecipitation method and SDS-PAGE, the molecular size of the antigen recognized by N-A8 was 210 kDa. The antigen was then purified by affinity chromatography and identified as neural adhesion molecule L1 by amino acid sequence analysis. By the present investigation, it was clearly demonstrated that L1 is expressed in human lung cancer cells. |
| Databáze: | OpenAIRE |
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