RNA-sequence analysis of gene expression from honeybees (Apis mellifera) infected with Nosema ceranae

Autor: Franco Mutinelli, Elisabetta Giuffra, Andre Fougeroux, Giovanni Formato, Giusy Cardeti, Anna Granato, Sara Botti, John L. Williams, A. Cersini, C. Gorni, Bouabid Badaoui, Fabien Petit, Anna Anselmo, Marco Cucurachi
Přispěvatelé: Integrative Biology Group, Centro Ricerche e Studi Agroalimentari (CeRSA), Science Faculty, Mohamed V University in Rabat, Syngenta France, RNAGRO, Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana, Istituto Zooprofilattico Sperimentale delle Venezie (IZSVe), Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Université Paris-Saclay, Davies Research Centre, University of Adelaide, Eureka-Eurostars Project Application [E!5069 NewBone], Syngenta, Botti, Sara, Istituto Zooprofilattico Sperimentale delle Venezie
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0106 biological sciences
0301 basic medicine
Time Factors
Odorant binding
[SDV]Life Sciences [q-bio]
lcsh:Medicine
Gene Expression
Genes
Insect
01 natural sciences
Post-Transcriptional Gene Regulation
apis mellifera
Nosema
Gene expression
Medicine and Health Sciences
Transcriptional regulation
Protein Isoforms
lcsh:Science
Promoter Regions
Genetic
Immune Response
Regulation of gene expression
Principal Component Analysis
Multidisciplinary
Reverse Transcriptase Polymerase Chain Reaction
Bees
Spores
Fungal
3. Good health
Insects
stress biotique
Host-Pathogen Interactions
parasite
Insect Proteins
Transcription Initiation Site
Honey Bees
Signal Transduction
Research Article
Arthropoda
DNA transcription
Immunology
Biology
Microbiology
03 medical and health sciences
nosema ceranae
Genetics
Parasitic Diseases
Animals
Gene Regulation
Gene
Sequence Analysis
RNA
Gene Expression Profiling
lcsh:R
Organisms
Biology and Life Sciences
biology.organism_classification
Invertebrates
Hymenoptera
Nosema ceranae
infection
Gene expression profiling
010602 entomology
030104 developmental biology
lcsh:Q
Energy Metabolism
Zdroj: PLoS ONE
PLoS ONE, Public Library of Science, 2017, 12 (3), ⟨10.1371/journal.pone.0173438⟩
Plos One 3 (12), . (2017)
PLoS ONE, Vol 12, Iss 3, p e0173438 (2017)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0173438⟩
Popis: Honeybees (Apis mellifera) are constantly subjected to many biotic stressors including parasites. This study examined honeybees infected with Nosema ceranae (N. ceranae). N. = ceranae infection increases the bees energy requirements and may contribute to their decreased survival. RNA-seq was used to investigate gene expression at days 5, 10 and 15 Post Infection (P. I) with N. ceranae. The expression levels of genes, isoforms, alternative transcription start sites (TSS) and differential promoter usage revealed a complex pattern of transcriptional and post-transcriptional gene regulation suggesting that bees use a range of tactics to cope with the stress of N. ceranae infection. N. ceranae infection may cause reduced immune function in the bees by: (i) disturbing the host amino acids metabolism (ii) down-regulating expression of antimicrobial peptides (iii) down-regulation of cuticle coatings and (iv) down-regulation of odorant binding proteins.
Databáze: OpenAIRE
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