Meiotic Chromosome Homology Search Involves Modifications of the Nuclear Envelope Protein Matefin/SUN-1
| Autor: | Edina Csaszar, Verena Jantsch, Pawel Pasierbek, Alexandra Penkner, Alexander Woglar, Alexandra Fridkin, Thomas Machacek, Yosef Gruenbaum, Gustav Ammerer, Antoine Baudrimont, Jiradet Gloggnitzer |
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| Rok vydání: | 2009 |
| Předmět: |
Chromosome movement
Nuclear Envelope Receptors Cytoplasmic and Nuclear CELLCYCLE Biology Microtubules Chromosomes General Biochemistry Genetics and Molecular Biology Homology (biology) Meiosis Serine Homologous chromosome Animals Meiotic Prophase I Phosphorylation Caenorhabditis elegans Caenorhabditis elegans Proteins Genetics Nucleoplasm Biochemistry Genetics and Molecular Biology(all) Synapsis Protein Structure Tertiary Checkpoint Kinase 2 Chromosome Pairing SIGNALING Cytoplasm Mutation CELLBIO SUN domain Protein Kinases |
| Zdroj: | Cell. 139:920-933 |
| ISSN: | 0092-8674 |
| DOI: | 10.1016/j.cell.2009.10.045 |
| Popis: | Summary Genome haploidization during meiosis depends on recognition and association of parental homologous chromosomes. The C. elegans SUN/KASH domain proteins Matefin/SUN-1 and ZYG-12 have a conserved role in this process. They bridge the nuclear envelope, connecting the cytoplasm and the nucleoplasm to transmit forces that allow chromosome movement and homolog pairing and prevent nonhomologous synapsis. Here, we show that Matefin/SUN-1 forms rapidly moving aggregates at putative chromosomal attachment sites in the meiotic transition zone (TZ). We analyzed requirements for aggregate formation and identified multiple phosphotarget residues in the nucleoplasmic domain of Matefin/SUN-1. These CHK-2 dependent phosphorylations occur in leptotene/zygotene, diminish during pachytene and are involved in pairing. Mimicking phosphorylation causes an extended TZ and univalents at diakinesis. Our data suggest that the properties of the nuclear envelope are altered during the time window when homologs are sorted and Matefin/SUN-1 aggregates form, thereby controling the movement, homologous pairing and interhomolog recombination of chromosomes. |
| Databáze: | OpenAIRE |
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