Detection of Respiratory Viruses and Subtype Identification of Influenza A Viruses by GreeneChipResp Oligonucleotide Microarray

Autor: Andrew T. Drysdale, Sean Conlan, David L. Hirschberg, Adolfo García-Sastre, Rachel Amos-Ritchie, David B. Boyle, Francisco Pozo, Inmaculada Casas, Jeffrey Hui, Neil Renwick, Philippa J. M. Jack, Gustavo Palacios, W. Ian Lipkin, Thomas Briese, Pilar Pérez-Breña, Vilma Savy, Phenix-Lan Quan, Elsa Baumeister, Omar Jabado, Kelly M. Lager, Jürgen A. Richt, Daniel Cisterna
Rok vydání: 2007
Předmět:
Zdroj: Journal of Clinical Microbiology
Journal of Clinical Microbiology, 2007, 45(8), 2359–2364.
Sistema de Gestión del Conocimiento ANLIS MALBRÁN
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron:ANLIS
ISSN: 0095-1137
DOI: 10.1128/jcm.00737-07
Popis: Acute respiratory infections are significant causes of morbidity, mortality, and economic burden worldwide. An accurate, early differential diagnosis may alter individual clinical management as well as facilitate the recognition of outbreaks that have implications for public health. Here we report on the establishment and validation of a comprehensive and sensitive microarray system for detection of respiratory viruses and subtyping of influenza viruses in clinical materials. Implementation of a set of influenza virus enrichment primers facilitated subtyping of influenza A viruses through the differential recognition of hemagglutinins 1 through 16 and neuraminidases 1 through 9. Twenty-one different respiratory virus species were accurately characterized, including a recently identified novel genetic clade of rhinovirus. Fil: Quan, Phenix-Lan. Columbia University. Jerome L. and Dawn Greene Infectious Disease Laboratory; Estados Unidos. Fil: Palacios, Gustavo. Columbia University. Jerome L. and Dawn Greene Infectious Disease Laboratory; Estados Unidos. Fil: Jabado, Omar J. Columbia University. Jerome L. and Dawn Greene Infectious Disease Laboratory; Estados Unidos. Fil: Conlan, Sean. Columbia University. Jerome L. and Dawn Greene Infectious Disease Laboratory; Estados Unidos. Fil: Hirschberg, David L. Stanford School of Medicine; Estados Unidos. Fil: Pozo, Francisco. Instituto de Salud Carlos III. Centro Nacional de Microbiología; España. Fil: Jack, Philippa J. M. Australian Animal Health Laboratory. CSIRO Livestock Industries; Australia. Fil: Cisterna, Daniel. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina. Fil: Renwick, Neil. Columbia University. Jerome L. and Dawn Greene Infectious Disease Laboratory; Estados Unidos. Fil: Hui, Jeffrey. Columbia University. Jerome L. and Dawn Greene Infectious Disease Laboratory; Estados Unidos. Fil: Drysdale, Andrew. Columbia University. Jerome L. and Dawn Greene Infectious Disease Laboratory; Estados Unidos. Fil: Amos-Ritchie, Rachel. Australian Animal Health Laboratory. CSIRO Livestock Industries; Australia. Fil: Baumeister, Elsa. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina. Fil: Savy, Vilma. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina. Fil: Lager, Kelly M. USDA. National Animal Disease Center; Estados Unidos. Fil: Richt, Jürgen A. USDA. National Animal Disease Center; Estados Unidos. Fil: Boyle, David B. Australian Animal Health Laboratory. CSIRO Livestock Industries; Australia. Fil: García-Sastre, Adolfo. Mount Sinai School of Medicine. Department of Microbiology and Emerging Pathogens Institute; Estados Unidos. Fil: Casas, Inmaculada. Instituto de Salud Carlos III. Centro Nacional de Microbiología; España. Fil: Perez-Breña, Pilar. Instituto de Salud Carlos III. Centro Nacional de Microbiología; España. Fil: Briese, Thomas. Columbia University. Jerome L. and Dawn Greene Infectious Disease Laboratory; Estados Unidos. Fil: Lipkin, W. Ian. Columbia University. Jerome L. and Dawn Greene Infectious Disease Laboratory; Estados Unidos.
Databáze: OpenAIRE