Lipidomics and RNA sequencing reveal a novel subpopulation of nanovesicle within extracellular matrix biomaterials
| Autor: | Vladimir A. Tyurin, Salma O. El-Mossier, Mark H. Murdock, Stephen F. Badylak, Yulia Y. Tyurina, Peter S. Timashev, George S. Hussey, Catalina Pineda Molina, Valerian E. Kagan, Madeline C. Cramer, Yoojin C. Lee |
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| Rok vydání: | 2020 |
| Předmět: |
Cell signaling
Liquid Phase Microextraction Sequence analysis Biocompatible Materials Extracellular vesicles 3T3 cells Extracellular matrix Extracellular Vesicles Mice 03 medical and health sciences 0302 clinical medicine Lipidomics medicine Animals Phospholipids Research Articles 030304 developmental biology 0303 health sciences Multidisciplinary Sequence Analysis RNA Chemistry Fatty Acids SciAdv r-articles Life Sciences RNA 3T3 Cells Tissue repair Extracellular Matrix Cell biology medicine.anatomical_structure Applied Sciences and Engineering 030220 oncology & carcinogenesis Nanoparticles Chromatography Liquid Subcellular Fractions Research Article |
| Zdroj: | Science Advances |
| ISSN: | 2375-2548 |
| DOI: | 10.1126/sciadv.aay4361 |
| Popis: | Matrix-bound nanovesicles (MBVs) are a novel subclass of extracellular vesicles. Biomaterials composed of extracellular matrix (ECM) provide both mechanical support and a reservoir of constructive signaling molecules that promote functional tissue repair. Recently, matrix-bound nanovesicles (MBVs) have been reported as an integral component of ECM bioscaffolds. Although liquid-phase extracellular vesicles (EVs) have been the subject of intense investigation, their similarity to MBV is limited to size and shape. Liquid chromatography–mass spectrometry (LC-MS)–based lipidomics and redox lipidomics were used to conduct a detailed comparison of liquid-phase EV and MBV phospholipids. Combined with comprehensive RNA sequencing and bioinformatic analysis of the intravesicular cargo, we show that MBVs are a distinct and unique subpopulation of EV and a distinguishing feature of ECM-based biomaterials. The results begin to identify the differential biologic activities mediated by EV that are secreted by tissue-resident cells and deposited within the ECM. |
| Databáze: | OpenAIRE |
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