FGF-2 signaling induces downregulation of TAZ protein in osteoblastic MC3T3-E1 cells
| Autor: | Kiyoshi Ohkawa, Katsuhiko Aoki, Keishi Marumo, Homare Eda, Katsuyuki Fujii |
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| Rok vydání: | 2008 |
| Předmět: |
musculoskeletal diseases
Cellular differentiation Biophysics Down-Regulation Biology Fibroblast growth factor Biochemistry 3T3 cells Mice Downregulation and upregulation Coactivator medicine Animals Molecular Biology Osteoblasts Proteins Cell Differentiation Osteoblast 3T3 Cells Cell Biology Cell biology RUNX2 medicine.anatomical_structure Fibroblast Growth Factor 2 Corepressor Acyltransferases Transcription Factors |
| Zdroj: | Biochemical and Biophysical Research Communications. 366:471-475 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2007.11.140 |
| Popis: | Transcriptional coactivator with PDZ-binding motif (TAZ) protein is a coactivator of Runx2 and corepressor of PPARgamma. It also induces differentiation of mesenchymal cells into osteoblasts. In this study, we found that FGF-2, which inhibits bone mineralization and stimulates cell proliferation, reduced the TAZ protein expression level in osteoblast-like cells, MC3T3-E1. This reduction was recovered by removing FGF-2 from the culture medium, which also restored the osteoblastic features of MC3T3-E1 cells. Furthermore, FGF-2-induced reduction of TAZ is blocked by a SAPK/JNK-specific inhibitor. These findings suggest that the expression of TAZ protein is involved in osteoblast proliferation and differentiation. This may help elucidate the discrepancies in the effect of FGF-2 and contribute to the understanding of FGF/FGFR-associated craniosynostosis syndrome etiology and treatment. |
| Databáze: | OpenAIRE |
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