Screening of Antimicrobial Resistance Genes and Epidemiological Features in Hospital and Community-Associated Carbapenem-Resistant Pseudomonas aeruginosa Infections
| Autor: | Ilknur Esen Yildiz, Songül Özyurt, Uğur Kostakoğlu, Erva Rakici, Nebahat Ejder, Ayşe Ertürk, Emine Sönmez, Ayşegül Çopur Çiçek |
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| Přispěvatelé: | RTEÜ, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Çopur, Ayşegül Çopur, Ertürk, Ayşe, Ejder, Nebahat, Rakıcı, Erva, Kostakoğlu, Uğur, Yıldız, İlknur Esen, Özyurt, Songül, Sönmez, Emine |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Epidemiology 030106 microbiology Biology medicine.disease_cause Microbiology Community associated 03 medical and health sciences 0302 clinical medicine antibiotic resistance genes Antibiotic resistance genes Pulsed-field gel electrophoresis medicine Pharmacology (medical) 030212 general & internal medicine Gene Original Research Pharmacology Pseudomonas aeruginosa PFGE biochemical phenomena metabolism and nutrition bacterial infections and mycoses Antimicrobial biology.organism_classification Infectious Diseases Infection and Drug Resistance Colistin epidemiology Bacteria medicine.drug |
| Zdroj: | Infection and Drug Resistance |
| ISSN: | 1178-6973 |
| Popis: | AyÅegül Çopur Çiçek,1 AyÅe Ertürk,2 Nebahat Ejder,1 Erva Rakici,1 UÄur KostakoÄlu,2 Ä°lknur Esen Yıldız,2 Songül Özyurt,3 Emine Sönmez2 1Recep Tayyip Erdogan University, Faculty of Medicine, Department of Medical Microbiology, Rize, Turkey; 2Recep Tayyip Erdogan University, Faculty of Medicine, Department of Infectious Diseases and Clinical Microbiology, Rize, Turkey; 3Recep Tayyip Erdogan University, Faculty of Medicine, Department of Chest Diseases, Rize, TurkeyCorrespondence: AyÅegül Çopur ÇiçekRecep Tayyip Erdogan University, Faculty of Medicine, Department of Medical Microbiology, Rize, 53100, TurkeyEmail acopurcicek@gmail.comIntroduction: Researching carbapenem-resistant isolates enables the identification of carbapenemase-producing bacteria and prevents their spread.Methods: P. aeruginosa isolates were recovered from Medicine Faculty of Recep Tayyip ErdoÄan University and identified by conventional methods and the automated Vitek 2 Compact system. Antimicrobial susceptibility experiments were performed in accordance with CLSI criteria and the automated Vitek 2 Compact system. The PCR method was investigated for the presence of β-lactamase resistance genes. PFGE typing was performed to show clonal relation among samples.Results: Seventy P. aeruginosa isolates were isolated from seventy patients. Of the patients, 67.1% had contact with the health service in the last 90 days and 75.7% of the patients had received antimicrobial therapy in the previous 90 days. Twenty-four isolates were carbapenem resistant, 2 isolates were multidrug-resistant except colistin, and none of the samples had colistin resistance. The gene encoding β-lactamase or metallo-β-lactamase was found in a total of 36 isolates. The blaVEB and blaPER genes were identified in 1 and 5 isolates alone or 17 and 13 isolates in combination with other resistance genes, respectively. The blaNDM was the most detected metallo-β-lactamase encoding gene (n=18), followed by blaKPC (n=12). blaIMP and blaVIM were detected in 5 and 1 isolates, respectively. Also, the association of blaVEB-blaPER and blaVEB-blaKPC-blaNDM was found to be very high. Much more resistance genes and co-occurrence were detected in hospital-acquired samples than community-acquired samples. No difference was found between the community and hospital-associated isolates according to PFGE results. Simultaneously from 6 patients, other microorganisms were also isolated and 5 of them died.Conclusion: The average length of stay (days) was found to be significantly higher in HAI group than CAI group. The death of 5 patients with fewer or no resistance genes showed that the co-existence of other microorganisms in addition to resistance genes was important on death.Keywords: Pseudomonas aeruginosa, antibiotic resistance genes, epidemiology, PFGE |
| Databáze: | OpenAIRE |
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