| Autor: |
Glenn M. Marshall, Belamy B. Cheung, Michael W. Parker, Giovanni Perini, Jamie I. Fletcher, Murray D. Norris, Michelle Haber, W. Clay Gustafson, Shizhen Zhu, Xiaoling Zhang, Tao Liu, Andrew J. Gifford, Matthias Fischer, Andre Oberthuer, Stefan Hüttelmaier, Larissa Doughty, Jessica L. Bell, Michael A. Gorman, Brendan W. Stevenson, Bryce Keenan, Chelsea Mayoh, Bing Liu, Joshua A. McCarroll, Jayne E. Murray, Giorgio Milazzo, Janith A. Seneviratne, Taylor Lim, Mika Herath, Olivia C. Ciampa, Daniel R. Carter, Hassina Massudi, Jessica K. Holien, Jessica Koach |
| Rok vydání: |
2023 |
| DOI: |
10.1158/0008-5472.22426177.v1 |
| Popis: |
Supplementary Figure S2. PA2G4 increases MYCN protein stability. A and B, Representative immunoblots from cycloheximide (CHX) chase assays measuring the half-life of MYCN protein after PA2G4 knockdown (A) or overexpression (B) in BE(2)-C and Kelly cells for 48 hours. Cells were then treated with 100 µg/µl CHX for up to 60 minutes followed by immunoblotting. C, Co-IP of total protein from BE(2)-C cells using IgG, anti-MYCN, anti-PA2G4 antibodies, followed by immunoblotting with anti-MYCN, anti-PA2G4, anti-AURKA, anti-Fbxw7 or anti-vinculin antibodies. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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