Proteome difference among the salivary proteins adsorbed onto metallic orthodontic brackets and hydroxyapatite discs
Autor: | Yizhi Xiao, Maria Pia Canales, Walter L. Siqueira, Lina María Marín, Karla Tonelli Bicalho Crosara |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
Bacterial Diseases Saliva Teeth Proteome Orthodontic Brackets Gene Expression Proteomics Biochemistry 0302 clinical medicine Medical Conditions Spectrum Analysis Techniques Caries Medicine and Health Sciences Chromatography High Pressure Liquid Multidisciplinary Chemistry Bracket Separation Processes Infectious Diseases Metals visual_art visual_art.visual_art_medium Medicine Structural Proteins Anatomy Research Article Spectrometry Mass Electrospray Ionization Science Oral Medicine Orthodontics Research and Analysis Methods Metal 03 medical and health sciences Adsorption X-ray photoelectron spectroscopy DNA-binding proteins Genetics Humans Gene Regulation Salivary Proteins and Peptides Distillation Biology and life sciences Mucin Proteins 030206 dentistry X-Ray Photoelectron Spectroscopy Regulatory Proteins 030104 developmental biology Durapatite Jaw Electron Beam Spectrum Analysis Techniques Digestive System Head Nuclear chemistry Transcription Factors |
Zdroj: | PLoS ONE, Vol 16, Iss 7, p e0254909 (2021) PLoS ONE |
ISSN: | 1932-6203 |
Popis: | The aim of this study was to investigate the atomic composition and the proteome of the salivary proteins adsorbed on the surface of orthodontic metallic bracket. For this, the atomic composition of orthodontic metallic brackets was analyzed with X-ray Photoelectron Spectroscopy (XPS). The acquired bracket pellicle was characterized after brackets were immersed in human whole saliva supernatant for 2 hours at 37°C. Hydroxyapatite (HA) discs were used as a control. Acquired pellicle was harvested from the HA discs (n = 12) and from the metallic brackets (n = 12). Proteomics based on mass spectrometry technology was used for salivary protein identification and characterization. Results showed that most of the proteins adsorbed on the surface of orthodontic metallic brackets and on the HA discs were identified specifically to each group, indicating a small overlapping between the salivary proteins on each study group. A total of 311 proteins present on the HA discs were unique to this group while 253 proteins were unique to metallic brackets, and only 45 proteins were common to the two groups. Even though most proteins were unique to each study group, proteins related to antimicrobial activity, lubrication, and remineralization were present in both groups. These findings demonstrate that the salivary proteins adsorbed on the bracket surface are dependent on the material molecular composition. |
Databáze: | OpenAIRE |
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