Proteome difference among the salivary proteins adsorbed onto metallic orthodontic brackets and hydroxyapatite discs

Autor: Yizhi Xiao, Maria Pia Canales, Walter L. Siqueira, Lina María Marín, Karla Tonelli Bicalho Crosara
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
Bacterial Diseases
Saliva
Teeth
Proteome
Orthodontic Brackets
Gene Expression
Proteomics
Biochemistry
0302 clinical medicine
Medical Conditions
Spectrum Analysis Techniques
Caries
Medicine and Health Sciences
Chromatography
High Pressure Liquid
Multidisciplinary
Chemistry
Bracket
Separation Processes
Infectious Diseases
Metals
visual_art
visual_art.visual_art_medium
Medicine
Structural Proteins
Anatomy
Research Article
Spectrometry
Mass
Electrospray Ionization
Science
Oral Medicine
Orthodontics
Research and Analysis Methods
Metal
03 medical and health sciences
Adsorption
X-ray photoelectron spectroscopy
DNA-binding proteins
Genetics
Humans
Gene Regulation
Salivary Proteins and Peptides
Distillation
Biology and life sciences
Mucin
Proteins
030206 dentistry
X-Ray Photoelectron Spectroscopy
Regulatory Proteins
030104 developmental biology
Durapatite
Jaw
Electron Beam Spectrum Analysis Techniques
Digestive System
Head
Nuclear chemistry
Transcription Factors
Zdroj: PLoS ONE, Vol 16, Iss 7, p e0254909 (2021)
PLoS ONE
ISSN: 1932-6203
Popis: The aim of this study was to investigate the atomic composition and the proteome of the salivary proteins adsorbed on the surface of orthodontic metallic bracket. For this, the atomic composition of orthodontic metallic brackets was analyzed with X-ray Photoelectron Spectroscopy (XPS). The acquired bracket pellicle was characterized after brackets were immersed in human whole saliva supernatant for 2 hours at 37°C. Hydroxyapatite (HA) discs were used as a control. Acquired pellicle was harvested from the HA discs (n = 12) and from the metallic brackets (n = 12). Proteomics based on mass spectrometry technology was used for salivary protein identification and characterization. Results showed that most of the proteins adsorbed on the surface of orthodontic metallic brackets and on the HA discs were identified specifically to each group, indicating a small overlapping between the salivary proteins on each study group. A total of 311 proteins present on the HA discs were unique to this group while 253 proteins were unique to metallic brackets, and only 45 proteins were common to the two groups. Even though most proteins were unique to each study group, proteins related to antimicrobial activity, lubrication, and remineralization were present in both groups. These findings demonstrate that the salivary proteins adsorbed on the bracket surface are dependent on the material molecular composition.
Databáze: OpenAIRE
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