Association between E-selectin expression and histopathological modification of glomerular lesions by non-nephritogenic IgM antibodies in experimental lupus nephritis
| Autor: | Miho Terada, Noboru Konishi, Hirokazu Sakan, Masayuki Iwano, Kimihiko Nakatani, Shuhei Yoshimoto, Osamu Asai, Masato Nose |
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| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
Pathology Mice Inbred MRL lpr Igm antibody medicine.medical_treatment Intraperitoneal injection Kidney Glomerulus Lupus nephritis Clone (cell biology) Mice SCID urologic and male genital diseases Mice Rheumatology E-selectin medicine Bystander effect Animals skin and connective tissue diseases Systemic lupus erythematosus biology business.industry medicine.disease Lupus Nephritis Immunoglobulin M Immunology biology.protein Histopathology business E-Selectin |
| Zdroj: | Modern rheumatology. 24(5) |
| ISSN: | 1439-7609 |
| Popis: | To examine the role played by E-selectin in bystander IgM-mediated modification of glomerular lesions in experimental lupus nephritis.Experimental lupus SCID mice were induced by an intraperitoneal injection of clone 7B6.8, which was derived from a MRL/lpr mouse and shown to induce wire-loop type glomerular lesions. Mice were subsequently administered clone Sp6, a non-nephritogenic IgM antibody- producing hybridoma. E-selectin expression was then evaluated in glomeruli showing histopathological conversion of lesions from wire-loop-like to a cell-proliferative form. We also investigated the effects of a circulating soluble form of E-selectin (sE-selectin) on the modification of glomerular lesions in this lupus model.In experimental lupus mice, glomerular E-selectin expression significantly increased during the conversion from wire-loop-like glomerular lesions to a cell-proliferative type mediated by a non-nephritogenic bystander IgM antibody in presence of a nephritogenic antibody. Intraglomerular infiltration of CD68 + macrophages correlated significantly with the glomerular level of E-selectin expression. In addition, overexpression of circulating sE-selectin significantly suppressed conversion to cell-proliferative glomerular lesions and glomerular macrophage infiltration in these lupus model mice.The histopathological modification of lupus nephritis by non-nephritogenic bystander IgM antibodies is associated in part with glomerular E-selectin expression. |
| Databáze: | OpenAIRE |
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