Optimization and Validation of a Sensitive HPLC–PDA Method for Simultaneous Determination of Torasemide and Spironolactone in Human Plasma using Central Composite Design
| Autor: | Sai Sandeep Mannemala, Venkatesan Subramanian, Kannappan Nagappan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Torasemide
Time Factors Resolution (mass spectrometry) Central composite design Bioanalytical method validation Analytical chemistry Spironolactone lcsh:Chemistry chemistry.chemical_compound Column liquid Chromatography Limit of Detection medicine Humans Acetonitrile Chromatography High Pressure Liquid General Environmental Science Detection limit Sulfonamides Chromatography Chemistry Central Composite Design Linearity Torsemide lcsh:QD1-999 Human plasma General Earth and Planetary Sciences Blood Chemical Analysis medicine.drug |
| Zdroj: | Acta Chimica Slovenica, Vol 62, Iss 3, Pp 633-641 (2015) |
| ISSN: | 1580-3155 1318-0207 |
| Popis: | A sensitive, accurate, precise and rapid HPLC-PDA method was developed and validated for the simultaneous determination of torasemide and spironolactone in human plasma using Design of experiments. Central composite design was used to optimize the method using content of acetonitrile, concentration of buffer and pH of mobile phase as independent variables, while the retention factor of spironolactone, resolution between torasemide and phenobarbitone; and retention time of phenobarbitone were chosen as dependent variables. The chromatographic separation was achieved on Phenomenex C(18) column and the mobile phase comprising 20 mM potassium dihydrogen ortho phosphate buffer (pH-3.2) and acetonitrile in 82.5:17.5 v/v pumped at a flow rate of 1.0 mL min(-1). The method was validated according to USFDA guidelines in terms of selectivity, linearity, accuracy, precision, recovery and stability. The limit of quantitation values were 80 and 50 ng mL(-1) for torasemide and spironolactone respectively. Furthermore, the sensitivity and simplicity of the method suggests the validity of method for routine clinical studies. |
| Databáze: | OpenAIRE |
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