Evaluation of serological assays available in a biosafety level 2 laboratory and their application for survey of Middle East respiratory syndrome coronavirus among livestock in Ethiopia
| Autor: | Shuetsu Fukushi, Yuma Hachiya, Takurou Teramichi, Hiroshi Sentsui, Keisuke Oguma, Simenew Keskes Melaku |
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| Rok vydání: | 2019 |
| Předmět: |
Camelus
camel 040301 veterinary sciences Middle East respiratory syndrome coronavirus viruses coronavirus medicine.disease_cause Virus Serology 0403 veterinary science 03 medical and health sciences Neutralization Tests Seroepidemiologic Studies Virology Biosafety level Chlorocebus aethiops medicine Animals Humans Vero Cells 030304 developmental biology Coronavirus 0303 health sciences Sheep Full Paper General Veterinary biology Goats Middle East respiratory syndrome Vesiculovirus 04 agricultural and veterinary sciences Containment of Biohazards medicine.disease biology.organism_classification serological survey HEK293 Cells Vesicular stomatitis virus Spike Glycoprotein Coronavirus Middle East Respiratory Syndrome Coronavirus biology.protein Cattle Ethiopia Antibody Coronavirus Infections |
| Zdroj: | Journal of Veterinary Medical Science The Journal of Veterinary Medical Science |
| ISSN: | 1347-7439 0916-7250 |
| DOI: | 10.1292/jvms.19-0436 |
| Popis: | A serological survey of Middle East respiratory syndrome coronavirus (MERS-CoV) was conducted among dromedary camels and herbivorous animals sharing the same pasturage in Ethiopia. The pseudotyped vesicular stomatitis virus coated with the spike protein of MERS-CoV was used in virus neutralization (VN) tests performed in a biosafety level (BSL)-2 laboratory. The results were similar to those obtained from the VN test using live MERS-CoV and were more sensitive than the ELISA performed using synthetic MERS S1 fragment as the antigen as well as the competitive ELISA performed using a monoclonal antibody against MERS-CoV. According to the comprehensive results of the four types of serodiagnosis methods, positive antibodies were detected only in dromedary camels and the remaining herbivorous animals were not infected with the virus. Moreover, using the present procedure, serological tests for MERS-CoV can be conducted even in BSL 2 laboratory. |
| Databáze: | OpenAIRE |
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