Expression of carotenoid biosynthesis genes during carrot root development
| Autor: | Jérémy Clotault, Mathilde Briard, Emmanuel Geoffriau, Mathieu Thomas, Didier Peltier, Romain Berruyer |
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| Přispěvatelé: | Institut National d'Horticulture, IFR 149 Quasav, UMR 1259 GenHort, F-49045 Angers, France, Génétique et Horticulture (GenHort), AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA), AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro) |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
0106 biological sciences
Lutein Carotenoid accumulation Transcription Genetic Physiology daucus-carota MESH: Plant Roots Plant Science Plant Roots 01 natural sciences chemistry.chemical_compound Gene Expression Regulation Plant Intramolecular Lyases Carotenoid Plant Proteins 2. Zero hunger chemistry.chemical_classification 0303 health sciences Phytoene synthase biology MESH: Plant Proteins plants food and beverages Lycopene yellow Daucus carota Biochemistry Geranylgeranyl-Diphosphate Geranylgeranyltransferase MESH: Biosynthetic Pathways Oxidoreductases Phytoene desaturase Zeaxanthin epoxidase cloning MESH: Alkyl and Aryl Transferases lycopene beta-cyclase [SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics 03 medical and health sciences MESH: Intramolecular Lyases Botany MESH: Daucus carota MESH: Oxidoreductases MESH: Gene Expression Regulation Plant development 030304 developmental biology ethylene regulation Alkyl and Aryl Transferases pathway organic chemicals MESH: Transcription Genetic tomato reveals fruit biology.organism_classification root Carotenoids Biosynthetic Pathways chemistry MESH: Carotenoids biology.protein gene expression Petal accumulation 010606 plant biology & botany |
| Zdroj: | Journal of Experimental Botany Journal of Experimental Botany, Oxford University Press (OUP), 2008, 59 (13), pp.3563-73. ⟨10.1093/jxb/ern210⟩ |
| ISSN: | 0022-0957 1460-2431 |
| DOI: | 10.1093/jxb/ern210⟩ |
| Popis: | Carotenogenesis has been extensively studied in fruits and flower petals. Transcriptional regulation is thought to be the major factor in carotenoid accumulation in these organs. However, little is known about regulation in root organs. The root carotenoid content of carrot germplasm varies widely. The present study was conducted to investigate transcriptional regulation of carotenoid biosynthesis genes in relation to carotenoid accumulation during early carrot root development and up to 3 months after sowing. HPLC carotenoid content analysis and quantitative RT-PCR were compared to quantify the expression of eight genes encoding carotenoid biosynthesis enzymes during the development of white, yellow, orange, and red carrot roots. The genes chosen encode phytoene synthase (PSY1 and PSY2), phytoene desaturase (PDS), zeta-carotene desaturase (ZDS1 and ZDS2), lycopene epsilon-cyclase (LCYE), lycopene beta-cyclase (LCYB1), and zeaxanthin epoxidase (ZEP). All eight genes were expressed in the white cultivar even though it did not contain carotenoids. By contrast with fruit maturation, the expression of carotenogenic genes began during the early stages of development and then progressively increased for most of these genes during root development as the total carotenoid level increased in coloured carrots. The high expression of genes encoding LCYE and ZDS noted in yellow and red cultivars, respectively, might be consistent with the accumulation of lutein and lycopene, respectively. The results showed that the accumulation of total carotenoids during development and the accumulation of major carotenoids in the red and yellow cultivars might partially be explained by the transcriptional level of genes directing the carotenoid biosynthesis pathway. |
| Databáze: | OpenAIRE |
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