An Efficient Vector System to Modify Cells Genetically
| Autor: | Xiaoli Liu, Wen He, Qingjun Liu, Bin Gao, Huamin Han, Kristy Ong |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Streptavidin
Clinical Research Design Genetic enhancement Genetic Vectors Immunology Cancer Treatment lcsh:Medicine Biology Transfection Biochemistry Microbiology Cell Line Green fluorescent protein chemistry.chemical_compound Genomic Medicine Cell Line Tumor Molecular Cell Biology Genetics Humans Biotinylation Carbon-Nitrogen Ligases lcsh:Science Gene Clinical Genetics Multidisciplinary lcsh:R fungi HEK 293 cells Gene targeting Human Genetics Gene Therapy Genomics Flow Cytometry Genetically modified organism Cell biology Oncology chemistry Medicine Electrophoresis Polyacrylamide Gel Clinical Immunology lcsh:Q Research Article Biotechnology |
| Zdroj: | PLoS ONE, Vol 6, Iss 11, p e26380 (2011) PLoS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0026380 |
| Popis: | The transfer of foreign genes into mammalian cells has been essential for understanding the functions of genes and mechanisms of genetic diseases, for the production of coding proteins and for gene therapy applications. Currently, the identification and selection of cells that have received transferred genetic material can be accomplished by methods, including drug selection, reporter enzyme detection and GFP imaging. These methods may confer antibiotic resistance, or be disruptive, or require special equipment. In this study, we labeled genetically modified cells with a cell surface biotinylation tag by co-transfecting cells with BirA, a biotin ligase. The modified cells can be quickly isolated for downstream applications using a simple streptavidin bead method. This system can also be used to screen cells expressing two sets of genes from separate vectors. |
| Databáze: | OpenAIRE |
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