A c-di-AMP riboswitch controlling kdpFABC operon transcription regulates the potassium transporter system in Bacillus thuringiensis

Autor: Xia Cai, Heon M. Lim, Hongdan Ma, Li Zhu, Shan-Ho Chou, Xun Wang, Jin He, Wen Yin, Xinfeng Li
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Communications Biology, Vol 2, Iss 1, Pp 1-10 (2019)
Communications Biology
ISSN: 2399-3642
Popis: The intracellular K+ level in bacteria is strictly controlled by K+ uptake and efflux systems. Among these, KdpFABC is a high-affinity K+ transporter system that is generally activated by the KdpDE two-component system in response to K+ limitation stress. However, the regulatory mechanism remains obscure in bacteria lacking the kdpDE genes. Here we report that the transcription of a kdpFABC operon is distinctively regulated by a cyclic diadenylate monophosphate (c-di-AMP) riboswitch located at the 5′-untranslated region of kdp transcript, and binding of c-di-AMP to the riboswitch promotes its intrinsic termination that blocks the kdpFABC transcription. Further, the intracellular c-di-AMP concentration was found to decrease under the K+ limitation stress, leading to transcriptional read-through over the terminator to allow kdpFABC expression. This regulatory element is found predominantly in the Bacillus cereus group and correlate well with the K+ and c-di-AMP homeostasis that affects a variety of crucial cellular functions.
Xun Wang et al. show a ydaO riboswitch-regulated kdp operon expression in Bacillus thuringiensis BMB171. This work experimentally validates a long-suspected connection between the kdpFABC operon and the ydaO riboswitch identified on the genome of BMB171.
Databáze: OpenAIRE
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