Abnormalities in the Pulmonary Innate Immune System in Cystic Fibrosis
| Autor: | Carola Loeve, Vera Cherepanov, Sergio Grinstein, Joanna H. Zurawska, Eric Vachon, Julianne V. Kus, Adeline Koh, Lori L. Burrows, Raiza Martin, Theo J. Moraes, Jenny Jongstra-Bilen, Gregory P. Downey, Jonathan Plumb |
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| Rok vydání: | 2006 |
| Předmět: |
Pulmonary and Respiratory Medicine
Staphylococcus aureus Cystic Fibrosis Phagocyte Neutrophils Phagocytosis Clinical Biochemistry Bronchi Respiratory Mucosa Biology medicine.disease_cause Cystic fibrosis Exocytosis Microbiology Flow cytometry Escherichia coli otorhinolaryngologic diseases medicine Humans Molecular Biology Cells Cultured Respiratory Burst Microbial Viability Innate immune system medicine.diagnostic_test Pseudomonas aeruginosa Osmolar Concentration Epithelial Cells Articles Cell Biology medicine.disease Immunity Innate Respiratory burst medicine.anatomical_structure |
| Zdroj: | American Journal of Respiratory Cell and Molecular Biology. 34:364-374 |
| ISSN: | 1535-4989 1044-1549 |
| DOI: | 10.1165/rcmb.2005-0146oc |
| Popis: | Pulmonary infection is the dominant clinical feature of cystic fibrosis (CF), but the basis for this susceptibility remains incompletely understood. One hypothesis is that CF airway surface liquid (ASL) is abnormal and interferes with neutrophil function. To study this possibility, we developed an in vitro system in which we collected ASL from primary cultures of normal and CF airway epithelial cells. Microbial killing was less efficient when bacteria were incubated with neutrophils in the presence of ASL from CF epithelia compared with normal ASL. Antimicrobial functions of human neutrophils were assessed in ASL from CF and normal epithelia using a combination of quantitative bacterial culture, flow cytometry, and microfluorescence imaging. The results of these assays of neutrophil function were indistinguishable in CF and normal ASL. In contrast, the direct bactericidal activity of ASL to Escherichia coli and to clinical isolates of Staphylococcus aureus and Pseudomonas aeruginosa was substantially less in CF than in normal ASL, even when highly diluted in media of identical ionic strength. Together, these observations indicate that the antimicrobial properties of ASL in CF are compromised in a manner independent of ionic strength of the ASL, and that this effect is not mediated through a direct effect of the ASL on phagocyte function. |
| Databáze: | OpenAIRE |
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