Single cell RNA analysis identifies cellular heterogeneity and adaptive responses of the lung at birth
| Autor: | Anne-Karina T. Perl, Samriddha Ray, Yan Xu, Minzhe Guo, Sheila M. Bell, Mike Adam, S. Steven Potter, Parvathi Sudha, Hitesh Deshmukh, Jason J. Gokey, Jeffrey A. Whitsett, Yina Du |
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| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Cell type Science Cell General Physics and Astronomy Respiratory Mucosa 02 engineering and technology Biology Article General Biochemistry Genetics and Molecular Biology Mice 03 medical and health sciences Pregnancy Gene expression medicine Animals lcsh:Science skin and connective tissue diseases Lung Oligonucleotide Array Sequence Analysis Regulation of gene expression Multidisciplinary Sequence Analysis RNA Gene Expression Profiling Gene Expression Regulation Developmental RNA Epithelial Cells General Chemistry 021001 nanoscience & nanotechnology Adaptation Physiological Cell biology Mice Inbred C57BL Gene expression profiling 030104 developmental biology medicine.anatomical_structure Animals Newborn Respiratory Physiological Phenomena Unfolded Protein Response Female lcsh:Q sense organs Single-Cell Analysis Signal transduction 0210 nano-technology |
| Zdroj: | Nature Communications, Vol 10, Iss 1, Pp 1-16 (2019) Nature Communications |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-018-07770-1 |
| Popis: | The respiratory system undergoes a diversity of structural, biochemical, and functional changes necessary for adaptation to air breathing at birth. To identify the heterogeneity of pulmonary cell types and dynamic changes in gene expression mediating adaptation to respiration, here we perform single cell RNA analyses of mouse lung on postnatal day 1. Using an iterative cell type identification strategy we unbiasedly identify the heterogeneity of murine pulmonary cell types. We identify distinct populations of epithelial, endothelial, mesenchymal, and immune cells, each containing distinct subpopulations. Furthermore we compare temporal changes in RNA expression patterns before and after birth to identify signaling pathways selectively activated in specific pulmonary cell types, including activation of cell stress and the unfolded protein response during perinatal adaptation of the lung. The present data provide a single cell view of the adaptation to air breathing after birth. The respiratory system is transformed in terms of functional change at birth to adapt to breathing air. Here, the authors examine the molecular changes behind the first breath in the mouse by Drop-seq based RNA sequencing, identifying activation of the unfolded protein response as a perinatal adaptation of the lung. |
| Databáze: | OpenAIRE |
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