A strategy for construction of industrial strains of distiller's yeast

Autor:	N. N. Kartasheva, N. I. Sutsov, S. V. Benevolensky, S. I. Ibragimova, B. D. Efremov, D. G. Kozlov
Rok vydání:	1995
Předmět:	Genetics Mating type Strain (biology) Saccharomyces cerevisiae Bioengineering Biology biology.organism_classification Applied Microbiology and Biotechnology Yeast Transformation (genetics) Fermentation Expression cassette Ploidy Biotechnology
Zdroj:	Biotechnology and Bioengineering. 46:285-290
ISSN:	1097-0290 0006-3592
DOI:	10.1002/bit.260460312
Popis:	A procedure was developed for construction of industrial strains of distiller's yeast (Saccharomyces cerevisiae). It includes several steps: construction of congenic genetically marked haploid strains of opposite mating types starting from an industrial strain of hybrid nature, integrative transformation of the above haploid strains with a DNA fragment containing an expression cassette responsible for new technological facilities, and hybridization of transformants and isolation of final industrial homozygous strains under experimental conditions simulating commercial fermentation processes. This strategy permits the generation of strains that have desirable characteristics of traditional races of distiller's yeast along with new technological facilities determined by the particular expression cassette. Using this procedure, we have constructed an industrial strain with improved amylolytic activity. © 1995 John Wiley & Sons, Inc.
Databáze:	OpenAIRE
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