Crystallization and preliminary X-ray diffraction studies of cyanuric acid hydrolase fromAzorhizobium caulinodans
| Autor: | Seunghee Cho, Hideki Aihara, Ke Shi, Lawrence P. Wackett |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Stereochemistry
Molecular Sequence Data Biophysics chemistry.chemical_element Biochemistry Amidohydrolases Azorhizobium caulinodans law.invention chemistry.chemical_compound Bacterial Proteins X-Ray Diffraction Structural Biology law Barbiturase Hydrolase Genetics Organic chemistry Amino Acid Sequence Crystallization Triazine Barbituric acid biology Magnesium Condensed Matter Physics biology.organism_classification chemistry Crystallization Communications biological sciences health occupations bacteria Cyanuric acid |
| Zdroj: | Acta Crystallographica Section F Structural Biology and Crystallization Communications. 69:880-883 |
| ISSN: | 1744-3091 |
| DOI: | 10.1107/s1744309113017077 |
| Popis: | Cyanuric acid is synthesized industrially and forms during the microbial metabolism of s-triazine herbicides. Cyanuric acid is metabolized by some microorganisms via cyanuric acid hydrolase (CAH), which opens the s-triazine ring as a prelude to further metabolism. CAH is a member of the rare cyanuric acid hydrolase/barbiturase family. Here, the crystallization and preliminary X-ray diffraction analysis of CAH from Azorhizobium caulinodans are reported. CAH was cocrystallized with barbituric acid, a close analog of cyanuric acid that is a tight-binding competitive inhibitor. Crystals suitable for X-ray diffraction experiments were grown in conditions containing PEG 8K or magnesium sulfate as precipitants. An X-ray diffraction data set was collected from CAH-barbituric acid crystals to 2.7 Å resolution. The crystals were found to belong to space group I4₁22, with unit-cell parameters a = b = 237.9, c = 105.3 Å, α = β = γ = 90°. |
| Databáze: | OpenAIRE |
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