Sodium selenite induces apoptosis and inhibits autophagy in human synovial sarcoma cell line SW982 in vitro
| Autor: | Yong Song Cai, Jialin Zhu, Xiaoqing Wu, Le Yang, She Min Lu, Jian Sun, Peng Xu, Yuan‑Bo Li, Ke Xu |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cancer Research autophagy Sodium Cell chemistry.chemical_element synovial sarcoma Biochemistry sodium selenite 03 medical and health sciences Sarcoma Synovial 0302 clinical medicine Cell Line Tumor Genetics medicine Humans Viability assay Cytotoxicity Molecular Biology Chemistry Caspase 3 Autophagy apoptosis Articles Cell cycle Cell biology 030104 developmental biology medicine.anatomical_structure Oncology Proto-Oncogene Proteins c-bcl-2 Apoptosis Cell culture 030220 oncology & carcinogenesis Molecular Medicine Poly(ADP-ribose) Polymerases SW982 cell line |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 1791-2997 |
| Popis: | The present study aimed to examine the effects of sodium selenite on the SW982 human synovial sarcoma cell line in relation to cell viability, apoptosis and autophagy. The results indicated that sodium selenite reduced cell viability and induced apoptosis by activating caspase‑3 and members of the poly (ADP‑ribose) polymerase and Bcl‑2 protein families in SW982 cells. Furthermore, autophagy was also suppressed by sodium selenite treatment in SW982 cells, and apoptosis was upregulated in cells co‑treated with sodium selenite and the autophagy inhibitor 3‑methyladenine. By contrast, apoptosis was downregulated when sodium selenite was combined with rapamycin, an inducer of autophagy. The results indicated that autophagy may protect cells from the cytotoxicity of sodium selenite. The present study results demonstrated that sodium selenite induced apoptosis and inhibited autophagy and autophagy‑protected cells from death by antagonizing sodium selenite‑induced apoptosis in SW982 cells in vitro. |
| Databáze: | OpenAIRE |
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