Efficacy of L-proline administration on the early responses during cutaneous wound healing in rats
| Autor: | Lonchin Suguna, Arulanandham Mathew, Sankar Jamuna, Moorthy Ganeshkumar, Karuppanan Natarajan Madhukumar, Thangavel Ponrasu, Kuttalam Iyappan |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Male
medicine.medical_specialty Lipid Peroxides Proline Clinical Biochemistry Connective tissue Administration Oral Ascorbic Acid Pharmacology Administration Cutaneous Biochemistry Antioxidants Superoxide dismutase chemistry.chemical_compound Oral administration medicine Animals Rats Wistar Skin chemistry.chemical_classification Glutathione Peroxidase Wound Healing integumentary system biology business.industry Superoxide Dismutase Glutathione peroxidase Organic Chemistry Glutathione Ascorbic acid Catalase Surgery Rats medicine.anatomical_structure chemistry biology.protein Granulation Tissue Collagen business Wound healing |
| Zdroj: | Amino acids. 45(1) |
| ISSN: | 1438-2199 |
| Popis: | Proline (Pro) plays a versatile role in cell metabolism and physiology. Pro and hydroxypro are major imino acids present in collagen, an important connective tissue protein, essential for wound healing, which is a primary response to tissue injury. This study explains the role of l-pro on cutaneous wound healing in rats when administered both topically and orally. Open excision wounds were made on the back of rats, and 200 μl (200 mg) of pro was administered topically and orally once daily to the experimental rats until the wounds healed completely. The control wounds were left untreated. Granulation tissues formed were removed after day 4 and 8 of post excision wounding, and biochemical parameters such as total protein, collagen, hexosamine, and uronic acid were estimated. Levels of enzymatic and non-enzymatic antioxidants such as catalase, superoxide dismutase, glutathione peroxidase, ascorbic acid, and reduced glutathione were evaluated along with lipid peroxides in the granulation tissues. Tensile strength and period of epithelialization were also measured. It was observed that the treated wounds healed very fast as evidenced by augmented rates of epithelialization and wound contraction, which was also confirmed by histological examinations. The results strappingly authenticate the beneficial effects of the topical administration of l-proline in the acceleration of wound healing than the oral administration and control. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink