Enzymatic Activities and Gene Transcript Levels Associated with the Augmentation of Antioxidant Constituents during Drought Stress in Lettuce
| Autor: | Aika Yoshimoto, Yuichi Uno, Misaki Ishibashi, Ryohei Koyama, Hiromichi Itoh |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Antioxidant
dehydrin Oxygen radical absorbance capacity medicine.medical_treatment Plant Science Horticulture SB1-1110 Superoxide dismutase medicine Food science Gene ascorbate peroxidase chemistry.chemical_classification biology fungi plant factories food and beverages Plant culture APX superoxide dismutase oxygen radical absorbance capacity Enzyme chemistry Polyphenol biology.protein RNA-seq rhizosphere Peroxidase |
| Zdroj: | Horticulturae, Vol 7, Iss 444, p 444 (2021) Horticulturae Volume 7 Issue 11 |
| ISSN: | 2311-7524 |
| Popis: | Efficient cultivation methods were investigated to promote the branding of products in plant factories. Moderate stress can enhance plant constituents that are beneficial for human health, without reducing yield. Dehydration stress in lettuce rhizospheres increased some antioxidants, including L-ascorbic acid (AsA) and polyphenols. In this study, the major factors contributing to the augmentation of antioxidant constituents were investigated. The drought treatment resulted in increased hydrophilic oxygen radical absorbance capacity (ORAC) values but not hydrophobic ORAC values. Both activities of antioxidant enzymes (superoxide dismutase, SOD, and ascorbate peroxidase, APX) were elevated under drought conditions. RNA-seq analysis revealed 33 upregulated and 115 downregulated differentially expressed genes, and 40 gene ontology enrichment categories. A dehydrin gene was the most significant among the upregulated genes in response to drought stress. Dehydrin protects plant cells from dryness through multiple functions, such as radical scavenging and protection of enzymes. Real-time PCR validated the substantial increase in some dehydrin paralogs with root desiccation. In conclusion, the enhancement of antioxidant levels by drought stress is likely not due to the induction of antioxidant enzyme genes, but due to increased enzymic activities. These activities might be protected by dehydrins encoded by the upregulated paralogs under drought stress. |
| Databáze: | OpenAIRE |
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