Chronic Individual Housing-Induced Stress Decreased Expression of Catecholamine Biosynthetic Enzyme Genes and Proteins in Spleen of Adult Rats
| Autor: | Sladjana Dronjak, Ljubica Gavrilović, Natasa Spasojevic |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Male
medicine.medical_specialty Sympathetic Nervous System Tyrosine 3-Monooxygenase Neuroimmunomodulation Immunology Down-Regulation Spleen Dopamine beta-Hydroxylase Biology 03 medical and health sciences Catecholamines 0302 clinical medicine Endocrinology Catecholamines plasma Internal medicine medicine Animals RNA Messenger Rats Wistar Gene 030304 developmental biology Catecholamine synthesis 0303 health sciences Endocrine and Autonomic Systems Phenylethanolamine N-Methyltransferase Biosynthetic enzyme Rats Disease Models Animal Induced stress medicine.anatomical_structure Gene Expression Regulation Social Isolation Neurology Immune System Chronic Disease Catecholamine Stress Psychological 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Neuroimmunomodulation |
| ISSN: | 1423-0216 1021-7401 |
| DOI: | 10.1159/000290042 |
| Popis: | Objective: Social isolation is regarded as one of the most relevant causes of diseases in mammalian species. The activation of the sympathoneural system represents one of the key components of the stress response. The sympathetic nervous system is one of the major pathways involved in immune-neuroendocrine interactions. The aim of the present study was to determine plasma epinephrine and norepinephrine in individually housed rats, as well as to find out whether splenic gene expression of catecholamine synthesizing enzymes and their protein levels are affected by chronic psychosocial stress. Methods: Tyrosine hydroxylase (TH), dopamine-β-hydroxylase (DBH) and phenylethanolamine N-methyltransferase (PNMT) mRNA levels were quantified by quantitative real-time RT-PCR. The TH, DBH and PNMT immunoproteins were assayed by Western blot. Results: Chronic social isolation of adult male rats produced a significant increase in plasma catecholamine levels and a decrease in splenic TH mRNA, DBH mRNA and PNMT mRNA. Protein levels of TH, DBH and PNMT were also reduced. Conclusion: These results suggest that increased plasma catecholamines and decreased gene expression and protein levels of catecholamine biosynthetic enzymes in the spleen of chronically individually housed animals might reduce catecholamine synthesis, thus leaving the immunocompetent tissues depleted of catecholamines and consequently leading to an impairment of immune response. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|