Effects of protein kinase inhibitors and protein phosphatase inhibitors on cyclic AMP-dependent down-regulation of vesicular monoamine transport in pheochromocytoma PC12 cells
| Autor: | Naomi Minami, Takao Ueha, Reiko Matsumoto, Hiroyuki Hasegawa, Satoshi Onozawa, Kinji Kurihara, Nobuo Nakanishi |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Serotonin
Phosphatase Carbazoles Biophysics Down-Regulation Pheochromocytoma PC12 Cells Biochemistry Indole Alkaloids Amine transport Norepinephrine chemistry.chemical_compound Protein phosphorylation Ethers Cyclic Structural Biology Vesicular Biogenic Amine Transport Proteins Okadaic Acid Phosphoprotein Phosphatases Genetics Cyclic AMP Animals Biogenic Monoamines Phosphorylation Protein kinase A Oxazoles Protein Kinase Inhibitors Molecular Biology Glycoproteins Membrane Glycoproteins Secretory vesicle Chemistry Kinase Neuropeptides Membrane Transport Proteins Biological Transport Cell Biology Okadaic acid Rats Bucladesine Monoamine transport Marine Toxins K252a |
| Zdroj: | FEBS Letters. (3):411-414 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(95)00698-9 |
| Popis: | Cyclic AMP down-regulates vesicular monoamine transport in PC12 cells and thereby decreased catecholamine reuptake from the extracellular fluid. We examined the effects of protein kinase inhibitors and protein phosphatase inhibitors on this cAMP action. Treatment of cells with a protein kinase inhibitor, K252a, increased vesicular amine transport and cellular amine uptake, thereby antagonizing the regulatory action of cAMP. In contrast, a protein phosphatase inhibitor, okadaic acid, had the opposite effect on the amine transport, i.e. it enhanced the cAMP action. These results suggest the involvement of a protein phosphorylation process in the cAMP-dependent modulation of vesicular monoamine transport. |
| Databáze: | OpenAIRE |
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