Bcl-2 overexpression attenuates resveratrol-induced apoptosis in U937 cells by inhibition of caspase-3 activity
| Autor: | Yun-Jung Choi, Min Ho Suh, Taeg Kyu Kwon, Won-Ki Baek, Do Sik Min, Antonino Passaniti, Jong Soo Chang, Ju Hyung Woo, Jong-Wook Park, Ing Nyol Jin, Seong Il Suh, Young Han Lee |
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| Rok vydání: | 2001 |
| Předmět: |
Cancer Research
Programmed cell death Chromosomal Proteins Non-Histone Survivin Blotting Western Apoptosis Cytochrome c Group Caspase 3 Resveratrol Transfection Inhibitor of Apoptosis Proteins Viral Proteins chemistry.chemical_compound Bcl-2-associated X protein Cyclins Proto-Oncogene Proteins Stilbenes Humans bcl-2-Associated X Protein biology Phospholipase C gamma Cytochrome c Cell Cycle U937 Cells General Medicine Flow Cytometry Caspase Inhibitors Molecular biology Cyclin-Dependent Kinases Neoplasm Proteins Isoenzymes Proto-Oncogene Proteins c-bcl-2 chemistry Caspases Type C Phospholipases biology.protein Poly(ADP-ribose) Polymerases Microtubule-Associated Proteins |
| Zdroj: | Carcinogenesis. 22:1633-1639 |
| ISSN: | 1460-2180 |
| DOI: | 10.1093/carcin/22.10.1633 |
| Popis: | Resveratrol has been shown to induce anti-proliferation and apoptosis of human cancer cell lines. In the present study, we determined the effect of high intracellular levels of the anti-apoptosis protein Bcl-2 on caspase-3 activation, PLC-gamma1 degradation and cytochrome c release during resveratrol-induced apoptosis. For this, we used U937/vector and U937/Bcl-2 cells, which were generated by transfection of the cDNA of the Bcl-2 gene. As compared with U937/vector, U937/Bcl-2 cells exhibited a 4-fold greater expression of Bcl-2. Treatment with 60 or 100 microM resveratrol for 24 h produced morphological features of apoptosis and DNA fragmentation in U937/vector cells, respectively. This was associated with caspase-3 activation and PLC-gamma1 degradation. In contrast, resveratrol-induced caspase-3 activation and PLC-gamma1 degradation and apoptosis were significantly inhibited in U937/Bcl-2 cells. Bcl-2 overexpressing cells exhibited less cytochrome c release and sustained expression levels of the IAP proteins during resveratrol-induced apoptosis. In addition, these findings indicate that Bcl-2 inhibits resveratrol-induced apoptosis by a mechanism that interferes with cytochrome c release and activity of caspase-3 that is involved in the execution of apoptosis. |
| Databáze: | OpenAIRE |
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