Monogalactosyldiacylglycerol: An abundant galactosyllipid of Cirsium brevicaule A. GRAY leaves inhibits the expression of gene encoding fatty acid synthase
| Autor: | Ruwani N. Nugara, Yasuo Kamiyama, Hirosuke Oku, Naoyuki Taira, Masashi Inafuku, Kensaku Takara |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Response element Pharmaceutical Science Cirsium 03 medical and health sciences 0302 clinical medicine Cell Line Tumor Drug Discovery Humans Luciferase RNA Messenger Viability assay Liver X receptor Pharmacology Messenger RNA Molecular Structure biology Chemistry Galactolipids Lipogenesis Fatty Acids Promoter Fatty Acid Synthase Type I Fatty acid synthase 030104 developmental biology Liver Complementary and alternative medicine Biochemistry 030220 oncology & carcinogenesis biology.protein Molecular Medicine |
| Zdroj: | Phytomedicine. 23:509-516 |
| ISSN: | 0944-7113 |
| DOI: | 10.1016/j.phymed.2016.02.023 |
| Popis: | Background The leaves of Cirsium brevicaule A. GRAY (CL) significantly decreased hepatic lipid accumulation and the expression of fatty acid synthase gene ( FASN ) in mice. Purpose We aimed to purify and identify the active compound(s) from CL and determine the inhibitory mechanism of expression of FASN . Methods We purified monogalactosyldiacylglycerol (MGDG) from extracts of CL (CL-MGDG) and showed that it was the active CL component through analyses of its effects on the expression of genes of human breast cancer cell line, SKBR-3. Results The content and fatty acid composition of CL-MGDG are distinctly different from those of other vegetable-derived MGDGs. Treatment of SKBR-3 cells with MGDG decreased the level of FASN mRNA as well as the levels of mRNA encoding other protein involved in lipogenesis. Further, MGDG treatments significantly inhibited luciferase activities of constructs containing liver X receptor response element in FASN promoter region without altering the levels of mRNA encoding transcription factors. MGDG and the FASN inhibitor C75 decreased the viabilities of SKBR-3 cells in a concentration-dependent manner. CL-MGDG more potently inhibited cell viability than a commercial MGDG preparation. Conclusions CL represents a good source of glycoglycerolipids with potential as functional ingredients of food. |
| Databáze: | OpenAIRE |
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