Triticum aestivum WRAB18 functions in plastids and confers abiotic stress tolerance when overexpressed in Escherichia coli and Nicotiania benthamiana
| Autor: | Linsheng Zhang, Hao Liu, Xiaoyu Wang, Yane Zhang, Dapeng Zhang, Zhenqing Bai |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Salinity Plastid localization Agricultural Biotechnology Nicotiana benthamiana lcsh:Medicine Plant Science medicine.disease_cause 01 natural sciences Genetically Modified Plants Physical Chemistry Biochemistry Plant Growth Regulators Gene Expression Regulation Plant Plant Resistance to Abiotic Stress Plastids lcsh:Science Triticum Flowering Plants Plant Proteins Abiotic component Multidisciplinary biology Ecology Chemistry Plant Biochemistry Genetically Modified Organisms Physics food and beverages Classical Mechanics Agriculture Plants Plants Genetically Modified Adaptation Physiological Plant Physiology Physical Sciences Mechanical Stress Genetic Engineering Research Article Biotechnology Nicotiana Plant Cell Biology Microbiology Superoxide dismutase 03 medical and health sciences Osmotic Pressure Stress Physiological Plant-Environment Interactions Tobacco Osmotic Shock medicine Escherichia coli Plant Defenses Abiotic stress Plant Ecology lcsh:R fungi Ecology and Environmental Sciences Organisms Biology and Life Sciences Cell Biology Plant Pathology biology.organism_classification APX 030104 developmental biology Thermal Stresses Chemical Properties biology.protein lcsh:Q Plant Biotechnology 010606 plant biology & botany Abscisic Acid |
| Zdroj: | PLoS ONE PLoS ONE, Vol 12, Iss 2, p e0171340 (2017) |
| ISSN: | 1932-6203 |
| Popis: | WRAB18, an ABA-inducible protein belongs to the third family of late embryogenesis abundant (LEA) proteins which can be induced by different biotic or abiotic stresses. In the present study, WRAB18 was cloned from the Zhengyin 1 cultivar of Triticum aestivum and overexpressed in Escherichia coli to explore its effects on the growth of E. coli under different abiotic stresses. Results suggested the enhanced exhibition of tolerance of E. coli to these stresses. Meanwhile, the WRAB18-transgenic tobacco plants were obtained to analyze the stress-related enzymatic activities of ascorbate peroxidase (APX), peroxidase (POD) and superoxide dismutase (SOD), and to quantify the content of malonaldehyde (MDA) under osmotic stress, high salinity, and low and high temperature stress. The activities of APX, POD and SOD in the transgenic tobacco lines were higher while the content of MDA was lower than those of WT lines. Moreover, plastid localization of WRAB18 in Nicotiana benthamiana plasma cells were found fusing with GFP. In addition, purified WRAB18 protein protected LDH (Lactate dehydrogenase) enzyme activity in vitro from various stress conditions. In brief, WRAB18 protein shows protective action behaving as a "molecular shield" in both prokaryotic and eukaryotic cells under various abiotic stresses, not only during ABA stress. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|