Extreme calorie restriction and energy source starvation in Saccharomyces cerevisiae represent distinct physiological states
| Autor: | Madelon Dijk, Pascale Daran-Lapujade, Antonius J. A. van Maris, Johannes H. de Winde, Jack T. Pronk, Leonie G. M. Boender, Marinka J. H. Almering |
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| Rok vydání: | 2011 |
| Předmět: |
Energy-Generating Resources
Saccharomyces cerevisiae Proteins Saccharomyces cerevisiae Calorie restriction Down-Regulation Biology Population heterogeneity chemistry.chemical_compound Adenosine Triphosphate Gene Expression Regulation Fungal medicine Carbon starvation Heat shock Molecular Biology Caloric Restriction Oligonucleotide Array Sequence Analysis Starvation ATP synthase Gene Expression Profiling Cell Biology Retentostat biology.organism_classification Yeast Glucose OA-Fund TU Delft Biochemistry chemistry Reserve carbohydrates biology.protein medicine.symptom Energy source Adenosine triphosphate Biomarkers Heat-Shock Response |
| Zdroj: | Biochimica et Biophysica Acta. Molecular Cell Research, 1813 (12), 2011 |
| ISSN: | 0167-4889 0006-3002 |
| DOI: | 10.1016/j.bbamcr.2011.07.008 |
| Popis: | Cultivation methods used to investigate microbial calorie restriction often result in carbon and energy starvation. This study aims to dissect cellular responses to calorie restriction and starvation in Saccharomyces cerevisiae by using retentostat cultivation. In retentostats, cells are continuously supplied with a small, constant carbon and energy supply, sufficient for maintenance of cellular viability and integrity but insufficient for growth. When glucose-limited retentostats cultivated under extreme calorie restriction were subjected to glucose starvation, calorie-restricted and glucose-starved cells were found to share characteristics such as increased heat-shock tolerance and expression of quiescence-related genes. However, they also displayed strikingly different features. While calorie-restricted yeast cultures remained metabolically active and viable for prolonged periods of time, glucose starvation resulted in rapid consumption of reserve carbohydrates, population heterogeneity due to appearance of senescent cells and, ultimately, loss of viability. Moreover, during starvation, calculated rates of ATP synthesis from reserve carbohydrates were 2–3 orders of magnitude lower than steady-state ATP-turnover rates calculated under extreme calorie restriction in retentostats. Stringent reduction of ATP turnover during glucose starvation was accompanied by a strong down-regulation of genes involved in protein synthesis. These results demonstrate that extreme calorie restriction and carbon starvation represent different physiological states in S. cerevisiae. |
| Databáze: | OpenAIRE |
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