Transretinal degeneration in ageing human retina: a multiphoton microscopy analysis
| Autor: | James Edwards Morgan, N. Garrahan, Michael P. Fautsch, Douglas H. Johnson, Michael E. Boulton, Boris Hermann, M. Hernandez, Y. Lei |
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| Přispěvatelé: | Cardiff University, Mayo Clinic, University of Florida [Gainesville] (UF), University of Wales College of Medicine |
| Rok vydání: | 2010 |
| Předmět: |
Adult
Male Retinal Ganglion Cells Retinal degeneration Pathology medicine.medical_specialty Adolescent Cell Count Biology Retina Article Imaging Young Adult 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine medicine Humans Outer nuclear layer Cellular Senescence Aged Aged 80 and over Microscopy Confocal medicine.disease eye diseases Sensory Systems Ophthalmology medicine.anatomical_structure Retinal ganglion cell Ageing Nerve Degeneration Inner nuclear layer 030221 ophthalmology & optometry Female sense organs Neuron Cell aging 030217 neurology & neurosurgery |
| Zdroj: | British Journal of Ophthalmology British Journal of Ophthalmology, BMJ Publishing Group, 2010, 95 (5), pp.727. ⟨10.1136/bjo.2010.180869⟩ |
| ISSN: | 0007-1161 1468-2079 |
| DOI: | 10.1136/bjo.2010.180869 |
| Popis: | International audience; Abstract Purpose: Retinal cell remodelling has been reported as a consistent feature of ageing. However the degree to which this results in trans-retinal degeneration is unclear. To address this, we used multiphoton microscopy to quantify retinal degeneration in post-mortem human eyes of two age groups. Methods: Retinas from six young subjects (18-33 years old) and six aged subjects (74-90 years old) were prepared as wholemount preparation. All retina were stained with DAPI and imaged by multiphoton confocal microscopy to quantify neuron densities in the retinal ganglion cell layer (RGCL), inner nucleus layer (INL) and outer nucleus layer (ONL). Neurons were identified using automated cell identification. All retinas were imaged hydrated to minimise counting artefacts. Results: In both groups, 56% of the area within the central 4 mm eccentricity, and 27% of the area with eccentricity between 4 mm and 7 mm were imaged. Compared with young subjects, the peak RGCL neuron loss in the aged subjects was at 1mm eccentricity of 25.5%. In the INL and ONL, neuron density significantly decreased at 1-2 mm eccentricity (8.7%) and 0.5-4 mm eccentricity (15.6%) respectively (P < 0.05). The reduction in neuron density in the INL corresponded spatially to the region with the greatest neuron loss in the RGCL and ONL, Conclusions: This is the first study to correlate neurodegeneration in different populations of cells in the ageing retinas. Our data confirm that the greatest neuronal loss occurs in the RGCL and ONL in human ageing retinas, whereas the INL is relatively preserved. |
| Databáze: | OpenAIRE |
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