Transcriptome analysis of a Pseudomonas aeruginosa sn-glycerol-3-phosphate dehydrogenase mutant reveals a disruption in bioenergetics
| Autor: | Jon Shuman, Leslie P Carroll, Laura Silo-Suh, Austin Powers, Kenji Tabata, Sang-Jin Suh, Tyler X Giles |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Bioenergetics 030106 microbiology Mutant Glycerolphosphate Dehydrogenase Dehydrogenase medicine.disease_cause Microbiology 03 medical and health sciences Bacterial Proteins Stress Physiological medicine biology Pseudomonas aeruginosa Chemistry Gene Expression Profiling Pseudomonas Gene Expression Regulation Bacterial biology.organism_classification Citric acid cycle Phenotype Glycerol-3-phosphate dehydrogenase Energy Metabolism rpoS Gene Deletion Metabolic Networks and Pathways |
| Zdroj: | Microbiology. 164:551-562 |
| ISSN: | 1465-2080 1350-0872 |
| DOI: | 10.1099/mic.0.000646 |
| Popis: | Pseudomonas aeruginosa causes acute and chronic human infections and is the major cause of morbidity and mortality in cystic fibrosis (CF) patients. We previously determined that the sn-glycerol-3-phosphate dehydrogenase encoded by glpD plays a larger role in P. aeruginosa physiology beyond its role in glycerol metabolism. To better understand the effect of a glpD mutation on P. aeruginosa physiology we compared the transcriptomes of P. aeruginosa strain PAO1 and the PAO1ΔglpD mutant using RNA-seq analysis. We determined that a null mutation of glpD significantly altered amino acid metabolism in P. aeruginosa and affected the production of intermediates that are channelled into the tricarboxylic acid cycle. Moreover, the loss of glpD induced a general stress response mediated by RpoS in P. aeruginosa. Several other phenotypes observed for the P. aeruginosa glpD mutant include increased persister cell formation, reduced extracellular ATP accumulation and increased heat output. Taken together, these findings implicate sn-glycerol-3-phosphate dehydrogenase as a key player in energy metabolism in P. aeruginosa. |
| Databáze: | OpenAIRE |
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