MicroRNA-24-3p alleviates hepatic ischemia and reperfusion injury in mice through the repression of STING signaling
| Autor: | Daofeng Zheng, Ai Shen, Qing-Song Chen, Zhongjun Wu, Yunhai Luo, Zuotian Huang, Tong Mou |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_treatment Biophysics Apoptosis Inflammation Pharmacology Liver transplantation Biochemistry Proinflammatory cytokine Mice 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Animals Medicine Molecular Biology business.industry Liver Diseases Membrane Proteins Cell Biology medicine.disease eye diseases MicroRNAs Sting 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation Liver Reperfusion Injury 030220 oncology & carcinogenesis Stimulator of interferon genes Hepatocyte Disease Progression Interferon Regulatory Factor-3 medicine.symptom business Reperfusion injury |
| Zdroj: | Biochemical and Biophysical Research Communications. 522:47-52 |
| ISSN: | 0006-291X |
| Popis: | Ischemia and reperfusion (I/R) injury is a common cause of hepatocyte injury and liver dysfunction during liver transplantation, but its mechanism is needed further explored. We aimed to investigate whether STING pathway activation is involved in the liver I/R and further determine the role of the microRNA(miR)-24-3p in liver I/R injury in mice. Our data showed that STING mRNA level was negatively related with miR-24-3p in livers of I/R-treated mice. Next, we identified that STING could be bound by miR-24-3p by bioinformatic and luciferase report assay. Moreover, downregulation of STING alleviated the protein expression of p-IRF3 and the serum level of inflammatory factor and aminotransferase in I/R mice model. Furthermore, transfection of I/R treated mice with exogenous miR-24-3p significantly inhibited the protein expression of STING and p-IRF3 in liver, and attenuated serum inflammatory cytokines release, as well as the dysfunction and apoptosis of liver in I/R model in vivo. This study suggests that miR-24-3p may ameliorate inflammatory response and cellular apoptosis in hepatic I/R process by targeting STING, which might be a potential therapeutic target for preventing liver I/R development and progression. |
| Databáze: | OpenAIRE |
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