Density-Dependent Induction of Apoptosis by Transforming Growth Factor-β1 in a Human Ovarian Carcinoma Cell Line
| Autor: | A. Moisand, Philippe Mazars, S. Jozan, M.G. Côme, C. Mathieu, Annie Valette |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Programmed cell death
endocrine system diseases Apoptosis Cell Count Adenocarcinoma Transforming Growth Factor beta Tumor Cells Cultured Humans Secretion Fragmentation (cell biology) Clonogenic assay Ovarian Neoplasms biology Cell growth DNA Neoplasm Cell Biology Transforming growth factor beta Molecular biology Chromatin female genital diseases and pregnancy complications Clone Cells Cell biology Cell culture Culture Media Conditioned biology.protein Female Cell Division |
| Zdroj: | Experimental Cell Research. 216:13-20 |
| ISSN: | 0014-4827 |
| Popis: | Transforming growth factor-beta 1 inhibited proliferation of a human ovarian carcinoma cell line (NIH-OVCAR-3). The inhibition of NIH-OVCAR-3 cell proliferation was accompanied by a decrease in clonogenic potential, evidenced by the reduced ability of TGF-beta 1-treated NIH-OVCAR-3 cells to form colonies on a plastic substratum. This rapid decrease of clonogenic potential, which was detected 6 h after addition of TGF-beta 1 was dose-dependent (IC50 = 4 pM). Fluorescence microscopy of DAPI-stained cells supported by electron-microscopic examination showed that TGF-beta 1 induced chromatin condensation and nuclear fragmentation. In addition, oligonucleosomal-sized fragments were detected in the TGF-beta 1-treated cells. These features indicated that TGF-beta 1 induced NIH-OVCAR-3 cell death by an apoptosis-like mechanism. This TGF-beta 1 apoptotic effect was subject to modulation by cell density. It was observed that an increase in cell density (up to 20 x 10(3) cells/cm2) protected NIH-OVCAR-3 cells against apoptosis induced by TGF-beta 1. Conditioned medium from high-density cultures of NIH-OVCAR-3 cells did not inhibit apoptosis induced by TGF-beta 1 on NIH-OVCAR-3 cells cultured at low density, suggesting that the protective effect of cell density was not related to the cell secretion of a soluble survival factor. |
| Databáze: | OpenAIRE |
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