Regulation of Apoptotic Endonucleases by EndoG
| Autor: | Tariq Fahmi, Eugene O. Apostolov, Xiaoying Wang, Dmitry D. Zhdanov, Alexei G. Basnakian, Sabzali Javadov, Sokolov Nn |
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| Rok vydání: | 2015 |
| Předmět: |
Original Research ArticlesOrganelles/Autophagy/Apoptosis
Blotting Western ENDOG Apoptosis DNA Fragmentation Real-Time Polymerase Chain Reaction Gene Expression Regulation Enzymologic Immunoenzyme Techniques Endonuclease chemistry.chemical_compound Image Processing Computer-Assisted In Situ Nick-End Labeling Genetics Animals Deoxyribonuclease I RNA Messenger Molecular Biology Gene Cells Cultured Deoxyribonucleases Endodeoxyribonucleases biology Reverse Transcriptase Polymerase Chain Reaction Apoptotic DNA fragmentation Brain Epithelial Cells Cell Biology General Medicine Flow Cytometry Molecular biology Rats Kidney Tubules chemistry biology.protein DNA fragmentation DNase I hypersensitive site Hypersensitive site DNA |
| Zdroj: | DNA and Cell Biology. 34:316-326 |
| ISSN: | 1557-7430 1044-5498 |
| DOI: | 10.1089/dna.2014.2772 |
| Popis: | Cells contain several apoptotic endonucleases, which appear to act simultaneously before and after cell death by destroying the host cell DNA. It is largely unknown how the endonucleases are being induced and whether they can regulate each other. This study was performed to determine whether apoptotic mitochondrial endonuclease G (EndoG) can regulate expression of other apoptotic endonucleases. The study showed that overexpression of mature EndoG in kidney tubular epithelial NRK-52E cells can increase expression of caspase-activated DNase (CAD) and four endonucleases that belong to DNase I group including DNase I, DNase X, DNase IL2, and DNase γ, but not endonucleases of the DNase 2 group. The induction of DNase I-type endonucleases was associated with DNA degradation in promoter/exon 1 regions of the endonuclease genes. These results together with findings on colocalization of immunostained endonucleases and TUNEL suggest that DNA fragmentation after EndoG overexpression was caused by DNase I endonucleases and CAD in addition to EndoG itself. Overall, these data provide first evidence for the existence of the integral network of apoptotic endonucleases regulated by EndoG. |
| Databáze: | OpenAIRE |
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