Phenylobacterium deserti sp. nov., isolated from desert soil
| Autor: | Mohammed A. M. Wadaan, Wael N. Hozzein, Inam Ullah Khan, Wan-Taek Im, Neeli Habib, Wen-Jun Li, Firasat Hussain, Xiao-Yang Zhi, Iftikhar Ahmed |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
DNA
Bacterial 0301 basic medicine food.ingredient Phenylobacterium composti Ubiquinone Phenylobacterium Orange (colour) Biology Microbiology 03 medical and health sciences food RNA Ribosomal 16S Botany Pakistan Phospholipids Phylogeny Soil Microbiology Ecology Evolution Behavior and Systematics Base Composition Oxidase test Fatty Acids Nucleic Acid Hybridization Caulobacteraceae Sequence Analysis DNA General Medicine 16S ribosomal RNA Terpenoid Bacterial Typing Techniques Phenylobacterium conjunctum genomic DNA 030104 developmental biology Desert Climate Glycolipids |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 67:4722-4727 |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijsem.0.002366 |
| Popis: | A novel bacterial strain, designated YIM 73061T, was isolated from the Cholistan desert in Punjab, Pakistan, and characterized by using a polyphasic taxonomic approach. 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to Phenylobacterium conjunctum FWC21T (97.6 %), Phenylobacterium lituiforme FaiI3T (97.4 %), Phenylobacterium composti 4T-6T (97.0 %) and Phenylobacterium aquaticum W2-3-4T (96.8 %). Cells were Gram-stain-negative, aerobic and motile rods that formed orange colonies. The strain was oxidase- and catalase-positive. Growth occurred at 20–40 °C (optimum, 30–37 °C) at pH 5.0–8.0 (optimum, pH 7.0) and with 0–1 % (w/v) NaCl (optimum, 0–0.5 %). The major cellular fatty acids (>10 %) were summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1ω6c) and C16 : 0. The polar lipid profile consisted of phosphatidylglycerol and four unidentified glycolipids. The major isoprenoid quinone was ubiquinone-10 (Q-10). The G+C content of the genomic DNA was 66.8 mol%. Strain YIM 73061T showed low levels of DNA–DNA relatedness to P. conjunctum FWC21T (27.2±2.6 %), P. lituiforme FaiI3T (24.6±1.1 %) and P. composti 4T-6T (18.4±3.1 %). On the basis of phylogenetic inference, chemotaxonomic characteristics and phenotypic data, strain YIM 73061T should be classified as representing a novel species, for which the name Phenylobacterium deserti sp. nov. is proposed. The type strain is YIM 73061T (=DSM 103871T=CCTCC AB 2016297T). |
| Databáze: | OpenAIRE |
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